Protein mixture analysis by MALDI/mobility/time-of-flight mass spectrometry

被引：5

作者：

Russell, DH ^{[1
]}

Gillig, KJ ^{[1
]}

Stone, E ^{[1
]}

Park, ZY ^{[1
]}

Fuhrer, K ^{[1
]}

Gonin, M ^{[1
]}

Schultz, AJ ^{[1
]}

机构：

[1] Texas A&M Univ, Dept Chem, Lab Biol Mass Spectrometry, College Stn, TX 77843 USA

来源：

ADVANCES IN NUCLEIC ACID AND PROTEIN ANALYSES, MANIPULATION, AND SEQUENCING | 2000年 / 1卷

关键词：

ion mobility; MALDI; protein digests; TOF; peptide/protein mixtures; drift tube;

D O I：

10.1117/12.380496

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Progress in the development of ion mobility (IM) orthogonal time-of-flight (oTOF) mass spectrometry for rapid analysis of biological samples is presented. The IM-oTOF apparatus described consists of a short drift tube (1 to 15 cm) designed for ion mobility measurement in the low-field limit and a low resolution linear (20 cm) TOF mass spectrometer. Proof of concept is demonstrated by analysis of peptide mixtures generated by proteolytic digestion of proteins.

引用

页码：69 / 78

页数：10

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