ASSESSING THE DIVERSITY OF BACTERIAL COMMUNITIES ASSOCIATED WITH PLANTS

被引:48
作者
Andreote, Fernando Dini [1 ,2 ]
Azevedo, Joao Lucio [1 ]
Araujo, Welington Luiz [1 ,3 ]
机构
[1] Univ Sao Paulo, Dept Genet, Escola Super Agr Luiz de Queiroz, BR-13400970 Sao Paulo, Brazil
[2] Embrapa Meio Ambiente, Lab Microbiol Ambiental, Jaguariuna, SP, Brazil
[3] Univ Mogi das Cruzes, Nucleo Integrado Biotecnol, Mogi Das Cruzes, SP, Brazil
关键词
Plant-bacteria interactions; molecular techniques; multivariate analysis; endophytes; rhizosphere; 16S RIBOSOMAL-RNA; GRADIENT GEL-ELECTROPHORESIS; POLYMERASE-CHAIN-REACTION; AMMONIA-OXIDIZING ARCHAEA; MICROBIAL COMMUNITY; GENETIC DIVERSITY; SOIL BACTERIA; MULTIVARIATE ANALYSES; RHIZOSPHERE BACTERIA; TRANSGENIC POTATOES;
D O I
10.1590/S1517-83822009000300001
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Plant-bacteria interactions result from reciprocal recognition between both species. These interactions are responsible for essential biological processes in plant development and health status. Here, we present a review of the methodologies applied to investigate shifts in bacterial communities associated with plants. A description of techniques is made from initial isolations to culture-independent approaches focusing on quantitative Polymerase Chain Reaction in real time (qPCR), Denaturing Gradient Gel Electrophoresis (DGGE), clone library construction and analysis, the application of multivariate analyses to microbial ecology data and the upcoming high throughput methodologies such as microarrays and pyrosequencing. This review supplies information about the development of traditional methods and a general overview about the new insights into bacterial communities associated with plants.
引用
收藏
页码:417 / 432
页数:16
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