Within-breed heterozygosity of canine single nucleotide polymorphisms identified by across-breed comparison

被引：6

作者：

Brouillette, JA

Venta, PJ ^{[1
]}

机构：

[1] Michigan State Univ, Coll Vet Med, E Lansing, MI 48824 USA

[2] Michigan State Univ, Coll Human Med, E Lansing, MI 48824 USA

[3] Michigan State Univ, Genet Program, E Lansing, MI 48824 USA

来源：

ANIMAL GENETICS | 2002年 / 33卷 / 06期

关键词：

Beagle; DNA pooling; Doberman Pinscher; inbreeding coefficient; Scottish terrier; single nucleotide polymorphisms;

D O I：

10.1046/j.1365-2052.2002.00918.x

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Identification of single nucleotide polymorphisms (SNPs) by DNA sequence comparison across breeds is a strategy for developing genetic markers that are useful for many breeds. However, the heterozygosity of SNPs identified in this way might be severely reduced within breeds by inbreeding or genetic drift in the small effective population size of a breed (population subdivision). The effect of inbreeding and population subdivision on heterozygosity of SNPs in dog breeds has never been investigated in a systematic way. We determined the genotypes of dogs from three divergent breeds for SNPs in four canine genes (ACTC, LMNA, SCGB, and TYMS) identified by across-breed DNA sequence comparison, and compared the genotype frequencies to those expected under Hardy-Weinberg equilibrium (HWE). Although population subdivision significantly skewed allele frequencies across breeds for two of the SNPs, the deviations of observed heterozygosities compared with those expected within breeds were minimal. These results indicate that across-breed DNA sequence comparison is a reasonable strategy for identifying SNPs that are useful within many canine breeds.

引用

页码：464 / 467

页数：4

共 8 条

[1] The essence of SNPs [J].