Distribution of Zn in functionally different leaf epidermal cells of the hyperaccumulator Thlaspi caerulescens

被引:156
作者
Frey, B
Keller, C
Zierold, K
Schulin, R
机构
[1] Swiss Fed Inst Forest Snow & Landscape Res, CH-8903 Birmensdorf, Switzerland
[2] Ecole Polytech Fed Lausanne, IGE Genie Sanit, CH-1015 Lausanne, Switzerland
[3] Max Planck Inst Mol Physiol, D-44227 Dortmund, Germany
[4] ETHZ, Inst Terr Ecol, CH-8952 Schlieren, Switzerland
关键词
Thlaspi caerulescens; cryo-ultramicrotomy; energy dispersive X-ray micro-analysis; high-pressure freezing; zinc;
D O I
10.1046/j.1365-3040.2000.00590.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The aim of this study was to show the potential of Thlaspi caerulescens in the cleaning-up of a moderately Zn-contaminated soil and to elucidate tolerance mechanisms at the cellular and subcellular level for the detoxification of the accumulated metal within the leaf. Measured Zn concentrations in shoots were high and reached a maximum value of 83 mmol kg(-1) dry mass, whereas total concentrations of Zn in the roots were lower (up to 13 mmol kg(-1)). In order to visualize and quantify Zn at the subcellular level in roots and leaves, ultrathin cryosections were analysed using energy-dispersive X-ray micro-analysis. Elemental maps of ultrathin cryosections showed that T. caerulescens mainly accumulated Zn in the vacuoles of epidermal leaf cells and Zn was almost absent from the vacuoles of the cells from the stomatal complex, thereby protecting the guard and subsidiary cells from high Zn concentrations. Observed patterns of Zn distribution between the functionally different epidermal cells were the same in both the upper and lower epidermis, and were independent of the total Zn content of the plant. Zinc stored in vacuoles was evenly distributed and no Zn-containing crystals or deposits were observed. From the elemental maps there was no indication that P, S or Cl was associated with the high Zn concentrations in the vacuoles. In addition, Zn also accumulated in high concentrations in both the cell walls of epidermal cells and in the mesophyll cells, indicating that apoplastic compartmentation is another important mechanism involved in zinc tolerance in the leaves of T. caerulescens.
引用
收藏
页码:675 / 687
页数:13
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