From glycomics to functional glycomics of sugar chains: Identification of target proteins with functional changes using gene targeting mice and knock down cells of FUT8 as examples

被引:25
作者
Kondo, Akihiro [1 ]
Li, Wenzhe
Nakagawa, Takatoshi
Nakano, Miyako
Koyama, Nobuto
Wang, Xiangchun
Gu, Jianguo
Miyoshi, Eiji
Taniguchi, Naoyuki
机构
[1] Osaka Univ, Grad Sch Med, Dept Glycotherapeut, Suita, Osaka 5650871, Japan
[2] Osaka Univ, Dept Dis Glycom, Res Inst Microbial Dis, Suita, Osaka 5650871, Japan
[3] Takara Bio Inc, Shiga 5202193, Japan
[4] Tohoku Pharmaceut Univ, Div Regulatory Glycobiol, Sendai, Miyagi 9818558, Japan
[5] Osaka Univ, Grad Sch Med, Dept Biochem, Suita, Osaka 5650871, Japan
来源
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS | 2006年 / 1764卷 / 12期
基金
日本科学技术振兴机构;
关键词
core fucosylation; functional glycomics; knock out; knock down; RNAi; lectin; MS;
D O I
10.1016/j.bbapap.2006.10.011
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Comprehensive analyses of proteins from cells and tissues are the most effective means of elucidating the expression patterns of individual disease-related proteins. On the other hand, the simultaneous separation and characterization of proteins by 1-DE or 2-DE followed by MS analysis are one of the fundamental approaches to proteomic analysis. However, these analyses do not permit the complete structural identification of glycans in glycoproteins or their structural characterization. Over half of all known proteins are glycosylated and glycan analyses of glycoproteins are requisite for fundamental proteomics studies. The analysis of glycan structural alterations in glycoproteins is becoming increasingly important in terms of biomarkers, quality control of glycoprotein drugs, and the development of new drugs. However, usual approach such as proteoglycomics, glycoproteomics and glycomics which characterizes and/or identifies sugar chains, provides some structural information, but it does not provide any information of functionality of sugar chains. Therefore, in order to elucidate the function of glycans, functional glycomics which identifies the target glycoproteins and characterizes functional roles of sugar chains represents a promising approach. In this review. we show examples of functional glycomics technique using alpha 1,6 fucosyltransferase gene (Fut8) in order to identify the target glycoprotein(s). This approach is based on glycan profiling by CE/MS and LC/MS followed by proteomic approaches, including 2-DE/1-DE and lectin blot techniques and identification of functional changes of sugar chains. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码:1881 / 1889
页数:9
相关论文
共 73 条
  • [1] PRODUCTION OF EMBRYONAL ALPHA-GLOBULIN BY TRANSPLANTABLE MOUSE HEPATOMAS
    ABELEV, GI
    PEROVA, SD
    KHRAMKOVA, NI
    POSTNIKOVA, ZA
    IRLIN, IS
    [J]. TRANSPLANTATION, 1963, 1 (02) : 174 - 180
  • [2] High resolution and high sensitivity methods for oligosaccharide mapping and characterization by normal phase high performance liquid chromatography following derivatization with highly fluorescent anthranilic acid
    Anumula, KR
    Dhume, ST
    [J]. GLYCOBIOLOGY, 1998, 8 (07) : 685 - 694
  • [3] CARBOHYDRATE STRUCTURES OF HUMAN ALPHA-FETOPROTEIN OF PATIENTS WITH HEPATOCELLULAR-CARCINOMA - PRESENCE OF FUCOSYLATED AND NONFUCOSYLATED TRIANTENNARY GLYCANS
    AOYAGI, Y
    SUZUKI, Y
    IGARASHI, K
    SAITOH, A
    OGURO, M
    YOKOTA, T
    MORI, S
    SUDA, T
    ISEMURA, M
    ASAKURA, H
    [J]. BRITISH JOURNAL OF CANCER, 1993, 67 (03) : 486 - 492
  • [4] FUCOSYLATION OF SERUM ALPHA-FETOPROTEIN IN PATIENTS WITH PRIMARY HEPATOCELLULAR-CARCINOMA
    AOYAGI, Y
    ISEMURA, M
    YOSIZAWA, Z
    SUZUKI, Y
    SEKINE, C
    ONO, T
    ICHIDA, F
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1985, 830 (03) : 217 - 223
  • [5] Use of targeted glycoproteomics to identify serum glycoproteins that correlate with liver cancer in woodchucks and humans
    Block, TM
    Comunale, MA
    Lowman, M
    Steel, LF
    Romano, PR
    Fimmel, C
    Tennant, BC
    London, WT
    Evans, AA
    Blumberg, BS
    Dwek, RA
    Mattu, TS
    Mehta, AS
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (03) : 779 - 784
  • [6] MICROHETEROGENEITY OF ALPHA-FETOPROTEIN IN PATIENT SERUM AS DEMONSTRATED BY LECTIN AFFINO-ELECTROPHORESIS
    BREBOROWICZ, J
    MACKIEWICZ, A
    BREBOROWICZ, D
    [J]. SCANDINAVIAN JOURNAL OF IMMUNOLOGY, 1981, 14 (01) : 15 - 20
  • [7] Conserved structural features in eukaryotic and prokaryotic fucosyltransferases
    Breton, C
    Oriol, R
    Imberty, A
    [J]. GLYCOBIOLOGY, 1998, 8 (01) : 87 - 94
  • [8] Therapeutic activity of humanized anti-CD20 monoclonal antibody and polymorphism in IgG Fc receptor FcγRIIIa gene
    Cartron, G
    Dacheux, L
    Salles, G
    Solal-Celigny, P
    Bardos, P
    Colombat, P
    Watier, H
    [J]. BLOOD, 2002, 99 (03) : 754 - 758
  • [9] Inhibitory Fc receptors modulate in vivo cytoxicity against tumor targets
    Clynes, RA
    Towers, TL
    Presta, LG
    Ravetch, JV
    [J]. NATURE MEDICINE, 2000, 6 (04) : 443 - 446
  • [10] DEUTSCH HF, 1991, ADV CANCER RES, V56, P253