Calnexin, calreticulin, and ERp57 cooperate in disulfide bond formation in human CD1d heavy chain

被引:91
作者
Kang, SJ [1 ]
Cresswell, P [1 ]
机构
[1] Yale Univ, Sch Med, Howard Hughes Med Inst, Immunobiol Sect, New Haven, CT 06520 USA
关键词
D O I
10.1074/jbc.M207831200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Members of the CD1 family of membrane glycoproteins can present antigenic lipids to T lymphocytes. Like major histocompatibility complex class I molecules, they form a heterodimeric complex of a heavy chain and beta(2)-microglobulin (beta(2)m) in the endoplasmic reticulum (ER). Binding of lipid antigens, however, takes place in endosomal compartments, similar to class II molecules, and on the plasma membrane. Unlike major histocompatibility complex class I or CD1b molecules, which need beta(2)m to exit the ER, CD1d can be expressed on the cell surface as either a free heavy chain or associated with beta(2)m. These differences led us to investigate early events of CD1d biosynthesis and maturation and the role of ER chaperones in its assembly. Here we show that CD1d associates in the ER with both calnexin and calreticulin and with the thiol oxidoreductase ERp57 in a manner dependent on glucose trimming of its N-linked glycans. Complete disulfide bond formation in the CD1d heavy chain was substantially impaired if the chaperone interactions were blocked by the glucosidase inhibitors castanospermine or N-butyldeoxynojirimycin. The formation of at least one of the disulfide bonds in the CD1d heavy chain is coupled to its glucose trimming-dependent association with ERp57, calnexin, and calreticulin.
引用
收藏
页码:44838 / 44844
页数:7
相关论文
共 59 条
[1]  
Amano M, 1998, J IMMUNOL, V161, P1710
[2]   A ROLE FOR CALNEXIN (IP90) IN THE ASSEMBLY OF CLASS-II MHC MOLECULES [J].
ANDERSON, KS ;
CRESSWELL, P .
EMBO JOURNAL, 1994, 13 (03) :675-682
[3]   Factors controlling the trafficking and processing of a leader-derived peptide presented by Qa-1 [J].
Bai, A ;
Aldrich, CJ ;
Forman, J .
JOURNAL OF IMMUNOLOGY, 2000, 165 (12) :7025-7034
[4]   The pathway for processing leader-derived peptides that regulate the maturation and expression of Qa-1b [J].
Bai, A ;
Broen, J ;
Forman, J .
IMMUNITY, 1998, 9 (03) :413-421
[5]   BETA(2)-MICROGLOBULIN-INDEPENDENT MHC CLASS IB MOLECULE EXPRESSED BY HUMAN INTESTINAL EPITHELIUM [J].
BALK, SP ;
BURKE, S ;
POLISCHUK, JE ;
FRANTZ, ME ;
YANG, L ;
PORCELLI, S ;
COLGAN, SP ;
BLUMBERG, RS .
SCIENCE, 1994, 265 (5169) :259-262
[6]   RECOGNITION OF A LIPID ANTIGEN BY CD1-RESTRICTED ALPHA-BETA(+) T-CELLS [J].
BEEKMAN, EM ;
PORCELLI, SA ;
MORITA, CT ;
BEHAR, SM ;
FURLONG, ST ;
BRENNER, MB .
NATURE, 1994, 372 (6507) :691-694
[7]   MONOCLONAL-ANTIBODY TO HLA-A3 [J].
BERGER, AE ;
DAVIS, JE ;
CRESSWELL, P .
HYBRIDOMA, 1982, 1 (02) :87-90
[8]   Intracellular trafficking pathway of newly synthesized CD1b molecules [J].
Briken, V ;
Jackman, RM ;
Dasgupta, S ;
Hoening, S ;
Porcelli, SA .
EMBO JOURNAL, 2002, 21 (04) :825-834
[9]   CD1d-mediated recognition of an α-galactosylceramide by natural killer T cells is highly conserved through mammalian evolution [J].
Brossay, L ;
Chioda, M ;
Burdin, N ;
Koezuka, Y ;
Casorati, G ;
Dellabona, P ;
Kronenberg, M .
JOURNAL OF EXPERIMENTAL MEDICINE, 1998, 188 (08) :1521-1528
[10]  
Brossay L, 1998, J IMMUNOL, V160, P3681