Quantifying Colorimetric Assays in Paper-Based Microfluidic Devices by Measuring the Transmission of Light through Paper

被引：323

作者：

Ellerbee, Audrey K. ^{[1
]}

Phillips, Scott T. ^{[1
]}

Siegel, Adam C. ^{[1
,2
]}

Mirica, Katherine A. ^{[1
]}

Martinez, Andres W. ^{[1
]}

Striehl, Pierre ^{[3
]}

Jain, Nina ^{[1
]}

Prentiss, Mara ^{[3
]}

Whitesides, George M. ^{[1
]}

机构：

[1] Harvard Univ, Dept Chem & Chem Biol, Cambridge, MA 02138 USA

[2] Harvard Univ, Sch Engn & Appl Sci, Cambridge, MA 02138 USA

[3] Harvard Univ, Dept Phys, Cambridge, MA 02138 USA

来源：

ANALYTICAL CHEMISTRY | 2009年 / 81卷 / 20期

关键词：

LOW-COST; HEALTH;

D O I：

10.1021/ac901307q

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This article describes a point-of-care (POC) system-comprising a microfluidic, paper-based analytical device (mu-PAD) and a hand-held optical colorimeter-for quantifying the concentration of analytes in biological fluids. The mu-PAD runs colorimetric assays, and consists of paper that has been (i) patterned to expose isolated regions of hydrophilic zones and (ii) wet with an index-matching fluid (e.g., vegetable oil) that is applied using a disposable, plastic sleeve encasement. Measuring transmittance through paper represents a new method of quantitative detection that expands the potential functionality of mu-PADs. This prototype transmittance colorimeter is inexpensive, rugged, and fully self-contained, and thus potentially attractive for use in resource-limited environments and developing countries.

引用

页码：8447 / 8452

页数：6

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