Sequence polymorphism and structural analysis of timothy grass pollen profilin allergen (Phl p 11)

被引：17

作者：

Asturias, JA

Arilla, MC

Bartolome, B

Martinez, J

Martinez, A

Palacios, R

机构：

[1] R and D Department, IFIDESA-ARISTEGUI, Bilbao

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1997年 / 1352卷 / 03期

关键词：

Phl p 11; sequence polymorphism; recombinant allergen; gene expression; timothy grass pollen; variant;

D O I：

10.1016/S0167-4781(97)00061-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Three cDNA clones encoding timothy grass pollen profilin (Phl p 11) were newly isolated. Comparison of the sequences of four cDNA clones, including a previously isolated clone, showed a low level of polymorphism. Isoelectrofocusing of highly purified timothy grass profilin indicated the existence of at least five isoforms. One recombinant profilin showed similar immunological properties to natural timothy grass profilin. Tertiary structure of Phleum pratense profilin was obtained by homology-based molecular modeling. (C) 1997 Elsevier Science B.V.

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页码：253 / 257

页数：5

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