Synthesis, binding and fluorescence properties of oligonucleotide derivatives having a dansyl fluorescence label attached to the 2'-position of a ribonucleoside

被引：34

作者：

Yamana, K

Ohashi, Y

Nunota, K

Nakano, H

机构：

[1] Department of Applied Chemistry, Himeji Institute of Technology, Himeji, Hyogo 671-22

来源：

TETRAHEDRON | 1997年 / 53卷 / 12期

关键词：

D O I：

10.1016/S0040-4020(97)00131-2

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Oligonucleotide derivatives having a dansyl fluorescence label at the 2'-position of a ribonucleoside have been synthesized by using 5'-dimethoxytrityl 2'-(dansylamino)uridine 3'-phosphorobisdethylamidie. The oligonucleotide containing a dansyl-modified uridine at the 5'-terminal position exhibits normal binding affinity for a complementary DNA segment in an aqueous buffer solution (pH 7.0). A significant increase in fluorescence intensity and blue-shift of the emission maximum were observed for dansyl-modified oligonucleotide upon binding to DNA. The fluorescence increase upon binding of dansyl-modified oligonucleotide to the target DNA was found to be sensitive to base mismatch in the DNA sequence. (C) 1997 Elsevier Science Ltd.

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页码：4265 / 4270

页数：6

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