Metabolic networks in motion:: 13C-based flux analysis

被引:477
作者
Sauer, Uwe [1 ]
机构
[1] ETH, Inst Mol Syst Biol, Wolfgang Pauli Str 16, CH-8093 Zurich, Switzerland
关键词
C-13; experiments; fluxome; metabolic engineering; metabolic pathways; network analysis;
D O I
10.1038/msb4100109
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Many properties of complex networks cannot be understood from monitoring the components - not even when comprehensively monitoring all protein or metabolite concentrations - unless such information is connected and integrated through mathematical models. The reason is that static component concentrations, albeit extremely informative, do not contain functional information per se. The functional behavior of a network emerges only through the nonlinear gene, protein, and metabolite interactions across multiple metabolic and regulatory layers. I argue here that intracellular reaction rates are the functional end points of these interactions in metabolic networks, hence are highly relevant for systems biology. Methods for experimental determination of metabolic fluxes differ fundamentally from component concentration measurements; that is, intracellular reaction rates cannot be detected directly, but must be estimated through computer model-based interpretation of stable isotope patterns in products of metabolism.
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页数:10
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