Identification of trypanosomes in wild animals from Southern Cameroon using the polymerase chain reaction (PCR)

被引:64
作者
Herder, S
Simo, G
Nkinin, S
Njiokou, F
机构
[1] CIRAD, LRCT, IRD, F-34398 Montpellier 5, France
[2] OCEAC, Lab Rech Trypanosomiases, Yaounde, Cameroon
[3] Univ Yaounde, Fac Sci, Yaounde, Cameroon
关键词
sleeping sickness; wild animal; reservoir; PCR; T; b; gambiense; forest belt; Cameroon;
D O I
10.1051/parasite/2002094345
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
One possible explanation of the maintenance of many historical foci of sleeping sickness in Central Africa could be the existence of a wild animal reservoir. In this study, PCR was used to detect the different trypanosome species present in wild animal captured by hunters in the southern forest belt of Cameroon (Bipindi). Trypanosomes were also detected by a parasitological method (Quantitative buffy coat QBC). Parasite could not be isolated in culture medium (Kit for in vitro isolation: KIVI). Specific primers of T. brucei s.l., T. congolense forest type, T. congolense savannah type, T vivax, T. simiae and T. b. gambiense group 1 were used to identify parasites in the blood of 164 animals belonging to 24 different species including ungulates, rodents, pangolins, carnivores, reptiles and primates. Of the 24 studied species, eight were carrying T. b. gambiense group 1. Those parasites pathogenic to man were found in monkeys (Cercocebus torquatus and Cercopithecus nictitans), in ungulates (Cephalophus dorsalis and C. monticola), in carnivores (Nandinia binotata and Genetta servalina) and in rodents (Cricetomys gambianus and Atherurus africanus). 13 species (54 %) were carrying T. brucei s.l. identified as non-gambiense group 1.
引用
收藏
页码:345 / 349
页数:5
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