Homocysteine promotes the LDL oxidase activity of ceruloplasmin

被引：21

作者：

Exner, M

Hermann, M

Hofbauer, R

Hartmann, B

Kapiotis, S

Gmeiner, B

机构：

[1] Univ Vienna, Dept Lab Med, Vienna, Austria

[2] Univ Vienna, Inst Mol Genet, Vienna, Austria

[3] Krankenhaus Neunkirchen, Inst Lab Med, Neukirchen, Austria

[4] Univ Vienna, Inst Med Chem, Vienna, Austria

来源：

FEBS LETTERS | 2002年 / 531卷 / 03期

关键词：

homocysteine; ceruloplasmin; copper ion; LDL oxidation;

D O I：

10.1016/S0014-5793(02)03571-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ceruloplasmin (CP) oxidises low density lipoprotein (LDL). The oxidising potential depends on the formation of Cu2+-CP which is redox-cycled during oxidation. Homocysteine (HCY) reduces free Cu2+, potentiating its cell-damaging property. We show that HCY enhanced LDL oxidation by CP, but did not activate the LDL oxidising potential of Cu2+-diamine oxidase. Selective removal of the redox-active Cu2+ abolished the LDL oxidase activity of CP. However, HCY partially restored the LDL oxidase activity of redox-copper depleted CP, indicating that the remaining six copper atoms in CP may also be involved in the process. Spectroscopic and oxidation inhibition studies using the Cu+-reagent bathocuproine revealed that HCY induced Cu+-CP formation, thus promoting its LDL oxidase activity. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

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页码：402 / 406

页数：5

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