A gag gene heteroduplex mobility assay for subtyping HIV-1

被引：24

作者：

Tatt, ID

Barlow, KL

Clewley, JP

机构：

[1] Cent Publ Hlth Lab, Hepatitis & Retrovirus Lab, London NW9 5HT, England

[2] Univ Cambridge, Dept Community Med, Inst Publ Hlth, Cambridge CB2 2SR, England

来源：

JOURNAL OF VIROLOGICAL METHODS | 2000年 / 87卷 / 1-2期

关键词：

gag gene; HMA subtyping; HIV-1;

D O I：

10.1016/S0166-0934(00)00146-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A heteroduplex mobility assay (HMA) using 753 and 446 base pair (bp) amplicons of the p17/p24 region of the gag gene of HIV-1 has been developed and validated with reference clones and clinical samples representative of subtypes A, B, C, D, E, G, and H. There was complete concordance between the gag HMA assigned subtype and the subtype known from gag or env sequence data or env HMA. The heteroduplexes from both amplicons can be clearly resolved on either MetaPhor XR agarose or MDE polyacrylamide gels. The MetaPhor XR gel system was the more convenient and is the preferred choice for routine HMA subtyping. This gag HMA provides a rapid, simple and inexpensive method for subtyping HIV-1 based on a genomic region other than the commonly used env gene target. The incorporation of gag HMA into subtype determination algorithms should allow the detection of gag/env recombinant strains of HIV-1. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：41 / 51

页数：11

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