Antibacterial effects of chitosan solutions® against Legionella pneumophila, Escherichia coli, and Staphylococcus aureus

被引:58
作者
Fujimoto, Takanori
Tsuchiya, Yasuo
Terao, Michinori
Nakamura, Kazutoshi
Yamamoto, Masaharu
机构
[1] Niigata Univ, Grad Sch Med & Dent Sci, Dept Community Prevent Med, Div Social & Environm Med, Niigata 9518510, Japan
[2] Niigata Univ, Fac Med, Sch Hlth Sci, Dept Med Technol, Niigata 9518518, Japan
关键词
antibacterial activity; ascorbic acid; lactic acid; Japan sea proper water; Legionella pneumophila; Escherichia coli;
D O I
10.1016/j.ijfoodmicro.2006.06.003
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Chitosan has been shown to have antibacterial activities on the growth of a wide variety of bacteria. Chitosan solution has been sold commercially for use as an antibacterial agent. Chitosan solution contains not only chitosan but also organic acids as solvents and desalted Japan Sea Proper Water (dJSPW). We aimed to clarify whether chitosan solution has antibacterial activity against bacteria invading bath water, and then to explore the causative factor among these ingredients. The antibacterial activity of full-strength chitosan solution and of 10(2)- and 10(4)-fold chitosan solution diluted with purified water was studied against Legionella pneumophila serogroups 1 (L. pneumophila SG1) and 6 (L. pneumophila SG6), Escherichia coli (E. coli), and Staphylococcus aureus (S. aureus) for 7 days at 37 degrees C. To clarify the causative factor in the antibacterial activity against E coli, the antibacterial activities of the full-strength and diluted chitosan solutions for 24 h were examined. L. pneumophila SG1 and SG6, and E coli could not survive in the chitosan solution or in the 10(2)-fold dilute solution for over a day at 37 degrees C. The cells of S. aureus were found to have decreased more than 2.46 log cfu/ml after 1 day of incubation, not only in the chitosan solutions, but also in phosphate buffer solution as a control. No inhibitory effect of dJSPW on the growth of the bacteria was observed. The antibacterial activity of the chitosan solution was lower compared with those of the organic acids solutions, and it increased with decreasing pH value. We observed the antibacterial activity of chitosan solution against L. pneumophila SG1 and SG6, and E. coli, suggesting it may be due to the decreased pH value derived from organic acids rather than from chitosan itself or dJSPW. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码:96 / 101
页数:6
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