Detection of the induction of Salmonella enterotoxin gene expression by contact with epithelial cells with RT-PCR

被引:19
作者
Dinjus, U
Hanel, I
Muller, W
Bauerfeind, R
Helmuth, R
机构
[1] FED INST HLTH PROTECT CONSUMERS & VET MED,DIV DIAG & EPIDEMIOL 5,D-12277 BERLIN,GERMANY
[2] UNIV GIESSEN,INST HYG & INFEKT KRANKHEITEN TIERE,D-35392 GIESSEN,GERMANY
关键词
Salmonella; epithelial cell; enterotoxin; gene expression;
D O I
10.1016/S0378-1097(96)00468-5
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
All strains of Salmonella enterica investigated were found to carry the Salmonella enterotoxin gene (stn) as determined by PCR and hybridization studies. However, when using CHO-K1 cells for testing the toxicity of the strains, not all strains showed a toxic effect (cell elongation) on the cells or did so only at a low level. The cultivation of Salmonella in contact with epithelial cells (IEC-6) led to an increase in the production of toxin. The sin gene expression was detectable with the help of the RT-PCR after 3 h of incubation. The RNA of the strains was isolated, transcribed into cDNA (with MMLV-reverse transcriptase) and amplified using PCR. The PCR products were separated electrophoretically using a polyacrylamide gel and detected by silver staining.
引用
收藏
页码:175 / 179
页数:5
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