Equilibration rates in lipid monolayers

A theoretical analysis is given for the rate of change of domain sizes in lipid monolayers at the air-water interface, The calculation is applicable to liquid domains formed from binary mixtures of lipids that form two coexisting liquid phases. Under conditions where the two lipid molecules have approximately equal areas, the equilibration rate does not involve macroscopic hydrodynamic flow in the subphase but rather depends on the diffusion coefficient of the lipid molecules. The calculation shows that the equilibration rate in binary mixtures of cholesterol and phosphatidylcholine is remarkably slow, the radius of a typical 20-mu m diameter domain changing by as little as a part In a million per second, Under these circumstances, equilibration times of the order of days or weeks are expected, Even with such long times, the final state reached by the monolayer will in general be a state of metastable equilibrium, rather than true equilibrium.