Significant levels of extracellular reactive oxygen species produced by brown rot basidiomycetes on cellulose

被引:70
作者
Cohen, R
Jensen, KA
Houtman, CJ
Hammel, KE [1 ]
机构
[1] Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA
[2] USDA, Forest Prod Lab, Madison, WI 53726 USA
关键词
wood decay; hydroxyl radical; aromatic hydroxylation; 2,5-dimethoxyhydroquinone; Gloeophyllum trabeum; Postia placenta;
D O I
10.1016/S0014-5793(02)03589-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
It is often proposed that brown rot basidiomycetes use extracellular reactive oxygen species (ROS) to accomplish the initial depolymerization of cellulose in wood, but little evidence has been presented to show that the fungi produce these oxidants in physiologically relevant quantities. We used [C-14]phenethyl polyacrylate as a radical trap to estimate extra-cellular ROS production by two brown rot fungi, Gloeophyllum trabeum and Postia placenta, that were degrading cellulose. Both fungi oxidized aromatic rings on the trap to give monohydroxylated and more polar products in significant yields. All of the cultures contained 2,5-dimethoxyhydroquinone, a fungal metabolite that has been shown to drive Fenton chemistry in vitro. These results show that extracellular ROS occur at significant levels in cellulose colonized by brown rot fungi, and suggest that hydroquinone-driven ROS production may contribute to decay by diverse brown rot species. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码:483 / 488
页数:6
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