Analysis of native human plasma proteins and haemoglobin for the presence of bityrosine by high-performance liquid chromatography

被引:20
作者
Daneshvar, B
Frandsen, H
Dragsted, AO
Knudsen, LE
Autrup, H
机构
[1] NATL INST OCCUPAT HLTH,COPENHAGEN,DENMARK
[2] AARHUS UNIV,DEPT ENVIRONM & OCCUPAT MED,AARHUS,DENMARK
来源
PHARMACOLOGY & TOXICOLOGY | 1997年 / 81卷 / 05期
关键词
D O I
10.1111/j.1600-0773.1997.tb00047.x
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Generation of reactive oxygen species in vivo results in oxidative-damage to cellular components, including proteins. Due to the relatively long half-lives of several blood proteins the cumulative formation of oxidatively damaged proteins might serve as a biomarker for reactive oxygen species formation. The most prominent sources of reactive oxygen species in vivo are site-specific metal ion-catalyzed reactions of the Fenton and Haber-Weiss types and the H2O2/peroxidase system. In vitro oxidation of L-tyrosine using a peroxidase or Cu++/H2O2 system gives rise to the formation of a highly fluorescent substance, bityrosine. High-performance liquid chromatography (HPLC) analysis of acid hydrolyzed serum albumin after oxidation with peroxidase/H2O2 or with Cu++/H2O2 showed that bityrosine had been formed whereas oxidation of this protein with Fe(III)/ascorbate did not result in the formation of bityrosine. Bityrosine could not be detected in human plasma proteins or haemoglobin with the detection limit of one pmol per mg protein.
引用
收藏
页码:205 / 208
页数:4
相关论文
共 16 条
[1]  
DAVIES KJA, 1987, J BIOL CHEM, V262, P9895
[2]  
DAVIES KJA, 1987, J BIOL CHEM, V262, P9908
[3]  
DAVIES KJA, 1987, J BIOL CHEM, V262, P9902
[4]  
DAVIES KJA, 1987, J BIOL CHEM, V262, P9914
[5]   TYROSINE-DERIVED CROSS-LINKING AMINO-ACIDS IN THE SHEATH OF HAEMONCHUS-CONTORTUS INFECTIVE LARVAE [J].
FETTERER, RH ;
RHOADS, ML .
JOURNAL OF PARASITOLOGY, 1990, 76 (05) :619-624
[6]   HYDROXYL RADICAL MEDIATED DAMAGE TO PROTEINS, WITH SPECIAL REFERENCE TO THE CRYSTALLINS [J].
GUPTASARMA, P ;
BALASUBRAMANIAN, D ;
MATSUGO, S ;
SAITO, I .
BIOCHEMISTRY, 1992, 31 (17) :4296-4303
[7]   DITYROSINE FORMATION IN THE PROTEINS OF THE EYE LENS [J].
GUPTASARMA, P ;
BALASUBRAMANIAN, D .
CURRENT EYE RESEARCH, 1992, 11 (11) :1121-1125
[8]  
HUGGINS TG, 1993, J BIOL CHEM, V268, P12341
[9]   FREE-RADICALS AS MEDIATORS OF TISSUE-INJURY AND DISEASE [J].
KEHRER, JP .
CRITICAL REVIEWS IN TOXICOLOGY, 1993, 23 (01) :21-48
[10]  
LUNEC J, 1987, FREE RADICALS OXIDAN, P151