Isolation of two novel genes, down-regulated in gastric cancer

被引：107

作者：

Yoshikawa, Y

Mukai, H

Hino, F

Asada, K

Kato, I

机构：

[1] Takara Shuzo Co Ltd, Adv Biomed Ctr, Shiga 525055, Japan

[2] Takara Shuzo Co Ltd, Biotechnol Res Labs, Otsu, Shiga 5202134, Japan

来源：

JAPANESE JOURNAL OF CANCER RESEARCH | 2000年 / 91卷 / 05期

关键词：

differential display; down-regulated expression; gastric cancer; digestive tract-specific calpain nCL-4;

D O I：

10.1111/j.1349-7006.2000.tb00967.x

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Using a differential display technique, we identified two genes that are down-regulated in human gastric cancer tissue as compared to normal gastric mucosa. The down-regulated expression of these genes in gastric cancer tissue was confirmed by northern blotting analysis and RT-PCR. One, CA11, was a novel gene expressed predominantly in the stomach and was depleted in all of the gastric cancer cell lines examined. The other gene, GC36, was homologous to the digestive tract-specific calpain gene, nCL-4, The expression of both GC36 and nCL-4 was suppressed or depleted in gastric cancer cell lines of differentiated and poorly differentiated types, This is the first report of genes, the expression of which is down-regulated with considerable frequency in gastric cancer.

引用

页码：459 / 463

页数：5

共 13 条

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