Oxidation of selenomethionine: some MADness in the method!

被引：24

作者：

Sharff, AJ

Koronakis, E

Luisi, B

Koronakis, V

机构：

[1] Univ Cambridge, Dept Biochem, Cambridge CB2 1GA, England

[2] Univ Cambridge, Dept Pathol, Cambridge CB2 1QP, England

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 2000年 / 56卷

关键词：

D O I：

10.1107/S090744490000370X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Since it was first reported, the multiwavelength anomalous diffraction (MAD) technique for the determination of protein structures has become widely accepted and increasingly popular. Here, it is demonstrated that the anomalous signal from selenomethione (SeMet) substituted proteins can be significantly enhanced by oxidation.

引用

页码：785 / 788

页数：4

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