P-, E-, and L-selectin mediate migration of activated CD8+ T lymphocytes into inflamed skin

被引:65
作者
Hirata, T
Furie, BC
Furie, B
机构
[1] Beth Israel Deaconess Med Ctr, Ctr Hemostasis & Thrombosis Res, Boston, MA 02215 USA
[2] Harvard Univ, Sch Med, Boston, MA 02215 USA
关键词
D O I
10.4049/jimmunol.169.8.4307
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
P- and E-selectin mediate CD4(+) Th1 cell migration into the inflamed skin in a murine contact hypersensitivity model. In this model, not only CD4(+) T cells but also CD8(+) T cells infiltrate the inflamed skin, and the role of CD8(+) type 1 cytotoxic T (Tc1) cells as effector cells has been demonstrated. Here we show that in mice deficient in both P- and E-selectin, the infiltration of CD8(+) T cells in the inflamed skin is reduced, suggesting the role of these selectins in CD8(+) T cell migration. We directly studied the role of selectins using in vitro-generated Tc1 cells. These cells are able to migrate into the inflamed skin of wild-type mice. This migration is partially mediated by P- and E-selectin, as shown by the reduced Tc1 cell migration into the inflamed skin of mice deficient in both P- and E-selectin or wild-type mice treated with the combination of anti-P-selectin and anti-E-selectin Abs. During P- and E-selectin-mediated migration of Tc1 cells, P-selectin glycoprotein ligand-1 appears to be the sole ligand for P-selectin and one of the ligands for E-selectin. P- and E-selectin-independent migration of Tc1 cells into the inflamed skin was predominantly. mediated by L-selectin. These observations indicate that all three selectins can mediate Tc1 cell migration into the inflamed skin.
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页码:4307 / 4313
页数:7
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