Nucleases Encoded by the Integrated Elements CJIE2 and CJIE4 Inhibit Natural Transformation of Campylobacter jejuni

被引：40

作者：

Gaasbeek, Esther J. ^{[1
,2
,3
]}

Wagenaar, Jaap A. ^{[1
,2
,3
,4
]}

Guilhabert, Magalie R. ^{[5
]}

van Putten, Jos P. M. ^{[3
,4
]}

Parker, Craig T. ^{[5
]}

van der Wal, Fimme J. ^{[1
,2
,4
]}

机构：

[1] Cent Vet Inst Wageningen UR, Dept Bacteriol, NL-8200 AB Lelystad, Netherlands

[2] Cent Vet Inst Wageningen UR, TSEs, NL-8200 AB Lelystad, Netherlands

[3] Univ Utrecht, Dept Infect Dis & Immunol, Utrecht, Netherlands

[4] WHO Collaborating Ctr Campylobacter, OIE Reference Lab Campylobacteriosis, Utrecht, Netherlands

[5] ARS, Produce Safety & Microbiol Res Unit, USDA, Albany, CA 94710 USA

来源：

JOURNAL OF BACTERIOLOGY | 2010年 / 192卷 / 04期

基金：

美国农业部;

关键词：

SERRATIA-MARCESCENS ENDONUCLEASE; HORIZONTAL GENE-TRANSFER; DIVERSITY; BACTERIA; SEQUENCE; DNASE; IDENTIFICATION; MUTAGENESIS; MECHANISM; STRAINS;

D O I：

10.1128/JB.00867-09

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The species Campylobacter jejuni is naturally competent for DNA uptake; nevertheless, nonnaturally transformable strains do exist. For a subset of strains we previously showed that a periplasmic DNase, encoded by dns, inhibits natural transformation in C. jejuni. In the present study, genetic factors coding for DNase activity in the absence of dns were identified. DNA arrays indicated that nonnaturally transformable dns-negative strains contain putative DNA/RNA nonspecific endonucleases encoded by CJE0566 and CJE1441 of strain RM1221. These genes are located on C. jejuni integrated elements 2 and 4. Expression of CJE0566 and CJE1441 from strain RM1221 and a homologous gene from strain 07479 in DNase-negative Escherichia coli and C. jejuni strains indicated that these genes code for DNases. Genetic transfer of the genes to a naturally transformable C. jejuni strain resulted in a decreased efficiency of natural transformation. Modeling suggests that the C. jejuni DNases belong to the Serratia nuclease family. Overall, the data indicate that the acquisition of prophage-encoded DNA/RNA nonspecific endonucleases inhibits the natural transformability of C. jejuni through hydrolysis of DNA.

引用

页码：936 / 941

页数：6

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