Effect in vitro of gamma interferon and interleukin-10 on generation of oxidizing species by human granulocytes

被引：32

作者：

Chaves, MM ^{[1
]}

Silvestrini, AA ^{[1
]}

SilvaTeixeira, DN ^{[1
]}

NogueiraMachado, JA ^{[1
]}

机构：

[1] UNIV FED MINAS GERAIS,INST CIENCIAS BIOL,DEPT BIOQUIM & IMUNOL,BR-30161970 BELO HORIZONT,MG,BRAZIL

来源：

INFLAMMATION RESEARCH | 1996年 / 45卷 / 07期

关键词：

human neutrophils; free radicals; reducing power; interleukin-10; gamma interferon;

D O I：

10.1007/BF02252942

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We examined the effect of interleukin-10 (IL-10), gamma interferon (IFN-gamma), phorbol ester (PDB), opsonized zymosan (OZ) and aminophylline (a cAMP phosphodiesterase inhibitor) on the reducing power and oxidizing species generation by human neutrophils, using MTT dye reduction and luminol-dependent chemiluminescence assays, respectively. Gamma interferon (IFN-gamma), phorbol ester (PDB) and opsonized zymosan (OZ) were activators while interleukin-10 (IL-10) and aminophylline were inhibitors. A strong parallelism was observed between oxidizing species generation and cellular reducing power in both activation and inhibition experiments. Our results also demonstrate for the first time the effect of IL-10 on free radical generation by neutrophils. The consequence of these activating and inhibiting effects on the inflammatory process are discussed.

引用

页码：313 / 315

页数：3

共 25 条

[1] EVIDENCE FOR GENERATION OF AN ELECTRONIC EXCITATIONS STATES) IN HUMAN POLYMORPHONUCLEAR LEUKOCYTES AND ITS PARTICIPATION IN BACTERICIDAL ACTIVITY [J].