Prevalence and occurrence of zoonotic bacterial pathogens in surface waters determined by quantitative PCR

被引:84
作者
Ahmed, W. [1 ,3 ]
Sawant, S. [2 ]
Huygens, F. [2 ]
Goonetilleke, A. [3 ]
Gardner, T. [1 ,3 ]
机构
[1] Dept Nat Resources & Water, Brisbane, Qld 4068, Australia
[2] Queensland Univ Technol, Sch Life Sci, Brisbane, Qld 4001, Australia
[3] Queensland Univ Technol, Sch Urban Dev, Brisbane, Qld 4001, Australia
关键词
Surface water; Fecal pollution; Zoonotic pathogens; Fecal indicators; Health risk; HUMAN FECAL POLLUTION; METABOLIC FINGERPRINT DATABASE; MICROBIAL SOURCE TRACKING; ESCHERICHIA-COLI O157-H7; 16S RIBOSOMAL-RNA; REAL-TIME PCR; ENVIRONMENTAL WATERS; CAMPYLOBACTER-JEJUNI; GENOTYPIC CHARACTERIZATION; INDICATOR ORGANISMS;
D O I
10.1016/j.watres.2009.03.041
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
The prevalence and concentrations of Campylobacter jejuni, Salmonella spp. and enterohaemorrhagic Escherichia coli (EHEC) were investigated in surface waters in Brisbane, Australia using quantitative PCR (qPCR) based methodologies. Water samples were collected from Brisbane City Botanic Gardens (CBG) Pond, and two urban tidal creeks (i.e., Oxley Creek and Blunder Creek). Of the 32 water samples collected, 8 (25%), 1 (3%), 9 (28%), 14 (44%), and 15 (47%) were positive for C. jejuni mapA, Salmonella invA, EHEC O157 LPS, EHEC VT1, and EHEC VT2 genes, respectively. The presence/absence of the potential pathogens did not correlate with either E. coli or enterococci concentrations as determined by binary logistic regression. In conclusion, the high prevalence, and concentrations of potential zoonotic pathogens along with the concentrations of one or more fecal indicators in surface water samples indicate a poor level of microbial quality of surface water, and could represent a significant health risk to users. The results from the current study would provide valuable information to the water quality managers in terms of minimizing the risk from pathogens in surface waters. (C) 2009 Published by Elsevier Ltd.
引用
收藏
页码:4918 / 4928
页数:11
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