Microchip electrophoresis-based separation of DNA

被引：77

作者：

Zhang, LH ^{[1
]}

Dang, FQ

Baba, Y

机构：

[1] Univ Tokushima, Fac Pharmaceut Sci, Dept Med Chem, CREST,Japan Sci & Technol Corp JST, Tokushima 7708505, Japan

[2] Furuno Elect Co Ltd, Nishinomiya, Hyogo, Japan

[3] Natl Inst Adv Ind Sci & Technol, AIST, Single Mol Bioanal Lab, Takamatsu, Kagawa 7610395, Japan

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2003年 / 30卷 / 06期

基金：

日本科学技术振兴机构;

关键词：

microchip electrophoresis; microfabrication; array electrophoresis microchip; genomics; review;

D O I：

10.1016/S0731-7085(02)00510-1

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Miniaturized instruments have developed very quickly in the last decade. This review is focused on the microchip electrophoresis-based separation of DNA. Fundamentals, including the chip format, substrates and fabrication technologies, fluid control, as well as various detection methods, are summarized. Array electrophoresis microchip and the on-chip integration of electrophoresis with other systems are introduced as well. In addition, the application of microchip electrophoresis in DNA sizing, genetic analysis and DNA sequencing are also presented in this paper. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：1645 / 1654

页数：10