Reversal of PCR bias for improved sensitivity of the DNA methylation melting curve assay

被引：51

作者：

Wojdacz, Tomasz K. ^{[1
]}

Hansen, Lise Lotte ^{[1
]}

机构：

[1] Univ Aarhus, Inst Human Genet, DK-8000 Aarhus C, Denmark

来源：

BIOTECHNIQUES | 2006年 / 41卷 / 03期

关键词：

D O I：

10.2144/000112240

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

[No abstract available]

引用

收藏

页码：274 / +

页数：3

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共 9 条

[1] Aggerholm A, 1999, CANCER RES, V59, P436
[2] Bisulfite conversion of genomic DNA for methylation analysis: protocol simplification with higher recovery applicable to limited samples and increased throughput
Boyd, VL
Zon, G
[J]. ANALYTICAL BIOCHEMISTRY, 2004, 326 (02) : 278 - 280
[3] Dobrovic A, 2005, MOL DIAGNOSTICS CLIN, P149
[4] A GENOMIC SEQUENCING PROTOCOL THAT YIELDS A POSITIVE DISPLAY OF 5-METHYLCYTOSINE RESIDUES IN INDIVIDUAL DNA STRANDS
FROMMER, M
MCDONALD, LE
MILLAR, DS
COLLIS, CM
WATT, F
GRIGG, GW
MOLLOY, PL
PAUL, CL
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (05) : 1827 - 1831
[5] GOTOH O, 1983, ADV BIOPHYS, V16, P1
[6] Profiling DNA methylation by melting analysis
Guldberg, P
Worm, J
Gronbæk, K
[J]. METHODS, 2002, 27 (02) : 121 - 127
[7] Methylation-specific PCR: A novel PCR assay for methylation status of CpG islands
Herman, JG
Graff, JR
Myohanen, S
Nelkin, BD
Baylin, SB
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (18) : 9821 - 9826
[8] Detection and measurement of PCR bias in quantitative methylation analysis of bisulphite-treated DNA
Warnecke, PM
Stirzaker, C
Melki, JR
Millar, DS
Paul, CL
Clark, SJ
[J]. NUCLEIC ACIDS RESEARCH, 1997, 25 (21) : 4422 - 4426
[9] Worm J, 2001, CLIN CHEM, V47, P1183