Bacterial secretome: the assembly manual and operating instructions (review)

被引:41
作者
Economou, A
机构
[1] Univ Crete, FORTH, Inst Mol Biol & Biotechnol, GR-71110 Iraklion, Greece
[2] Univ Crete, Dept Biol, GR-71110 Iraklion, Greece
关键词
protein translocase; chaperone; signal peptidase; SecA; SecYEG; SecDF; ATPase; motor protein; membrane transporter;
D O I
10.1080/09687680210152609
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Bacterial protein secretion is a complex multi-stage reaction that is central to membrane and cell wall biosynthesis and essential for cell viability. An impressive array of experimental tools have been used to dissect this reaction into discreet sub-reactions. Synthesis of these data reveals a fascinating cascade of inter- and. intra-molecular interactions that select, sort and target secretory polypeptides to the membrane and then spend metabolic energy to bias their vectorial movement across the membrane plane through a lipid-inaccessible proteinaceous environment. Transmembrane crossing is catalyzed by protein translocase, an astonishingly dynamic molecular machine. The unusual molecular features of the Sec pathway components allows a handful of proteins to catalyze the export of hundreds of secretory polypeptide substrates with astonishing fidelity. Knowledge of the molecular details of the secretion pathway allows us to rationally exploit these features in heterologous protein production biotechnologies and in the development of novel antibiotics.
引用
收藏
页码:159 / 169
页数:11
相关论文
共 133 条
  • [1] RECONSTITUTION OF A PROTEIN TRANSLOCATION SYSTEM CONTAINING PURIFIED SECY, SECE, AND SECA FROM ESCHERICHIA-COLI
    AKIMARU, J
    MATSUYAMA, SI
    TOKUDA, H
    MIZUSHIMA, S
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (15) : 6545 - 6549
  • [2] Identification and analysis of bacterial protein secretion inhibitors utilizing a SecA-LacZ reporter fusion system
    Alksne, LE
    Burgio, P
    Hu, W
    Feld, B
    Singh, MP
    Tuckman, M
    Petersen, PJ
    Labthavikul, P
    McGlynn, M
    Barbieri, L
    McDonald, L
    Bradford, P
    Dushin, RG
    Rothstein, D
    Projan, SJ
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2000, 44 (06) : 1418 - 1427
  • [3] A proteomic view on genome-based signal peptide predictions
    Antelmann, H
    Tjalsma, H
    Voigt, B
    Ohlmeier, S
    Bron, S
    van Dijl, JM
    Hecker, M
    [J]. GENOME RESEARCH, 2001, 11 (09) : 1484 - 1502
  • [4] SECD AND SECF ARE REQUIRED FOR THE PROTON ELECTROCHEMICAL GRADIENT STIMULATION OF PREPROTEIN TRANSLOCATION
    ARKOWITZ, RA
    WICKNER, W
    [J]. EMBO JOURNAL, 1994, 13 (04) : 954 - 963
  • [5] TRANSLOCATION CAN DRIVE THE UNFOLDING OF A PREPROTEIN DOMAIN
    ARKOWITZ, RA
    JOLY, JC
    WICKNER, W
    [J]. EMBO JOURNAL, 1993, 12 (01) : 243 - 253
  • [6] Recombinant protein expression in Escherichia coli
    Baneyx, F
    [J]. CURRENT OPINION IN BIOTECHNOLOGY, 1999, 10 (05) : 411 - 421
  • [7] BASSILANA M, 1992, J BIOL CHEM, V267, P25246
  • [8] Allosteric communication between signal peptides and the SecA protein DEAD motor ATPase domain
    Baud, C
    Karamanou, S
    Sianidis, G
    Vrontou, E
    Politou, AS
    Economou, A
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (16) : 13724 - 13731
  • [9] Berges H, 1996, APPL ENVIRON MICROB, V62, P55
  • [10] The SecYEG preprotein translocation channel is a conformationally dynamic and dimeric structure
    Bessonneau, P
    Besson, V
    Collinson, I
    Duong, F
    [J]. EMBO JOURNAL, 2002, 21 (05) : 995 - 1003