Assembly of light harvesting complexes II(LHC-II) in the absence of lutein - A study on the alpha-carotenoid-free mutant C-2A'-34 of the green alga Scenedesmus obliquus

被引：19

作者：

Heinze, I

Pfundel, E

Huhn, M

Dau, H

机构：

[1] UNIV MARBURG,FACHBEREICH BIOL BOT,D-35032 MARBURG,GERMANY

[2] INST PFLANZENGENET & KULTURPFLANZENFORSCH,D-06466 GATERSLEBEN,GERMANY

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS | 1997年 / 1320卷 / 02期

关键词：

carotenoid; chlorophyll; Photosystem II; photosynthesis;

D O I：

10.1016/S0005-2728(97)00022-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Stable assembly of the major LHC-II proteins (22, 26 kDa) is inhibited in the alpha-carotenoid-free mutant C-2A'-34 of the green alga Scenedesmus obliquus, whereas other LHC-II proteins (22, 23, 29 kDa) seem to be not affected. We conclude that lutein is essential for assembly of the major LHC-II proteins of S. obliquus, but not for assembly of the minor LHC-II proteins. It is proposed that in the minor LHC-II proteins lutein is replaced by violaxanthin, zeaxanthin and antheraxanthin.

引用

收藏

页码：188 / 194

页数：7

相关论文

共 24 条

[1] CHLOROPHYLL-PROTEIN COMPLEXES OF SPINACH AND BARLEY THYLAKOIDS - SPECTRAL CHARACTERIZATION OF 6 COMPLEXES RESOLVED BY AN IMPROVED ELECTROPHORETIC PROCEDURE [J].

ANDERSON, JM ;

WALDRON, JC ;

THORNE, SW .

FEBS LETTERS, 1978, 92 (02) :227-233

[2] COMPLETE SEPARATION OF THE BETA,EPSILON-CAROTENOID AND BETA,BETA-CAROTENOID BIOSYNTHETIC PATHWAYS BY A UNIQUE MUTATION OF THE LYCOPENE CYCLASE IN THE GREEN-ALGA, SCENEDESMUS-OBLIQUUS [J].

BISHOP, NI ;

URBIG, T ;

SENGER, H .

FEBS LETTERS, 1995, 367 (02) :158-162

[3]

BISHOP NI, 1995, PHOTOSYNTHESIS LIGHT, V4, P135

[4] INVITRO RECONSTITUTION OF A LIGHT-HARVESTING GENE-PRODUCT - DELETION MUTAGENESIS AND ANALYSES OF PIGMENT BINDING [J].

CAMMARATA, KV ;

SCHMIDT, GW .

BIOCHEMISTRY, 1992, 31 (10) :2779-2789

[5] Functional analysis of the beta and epsilon lycopene cyclase enzymes of Arabidopsis reveals a mechanism for control of cyclic carotenoid formation [J].

Cunningham, FX ;

Pogson, B ;

Sun, ZR ;

McDonald, KA ;

DellaPenna, D ;

Gantt, E .

PLANT CELL, 1996, 8 (09) :1613-1626

[6] CAROTENOIDS AND PHOTOPROTECTION IN PLANTS - A ROLE FOR THE XANTHOPHYLL ZEAXANTHIN [J].

DEMMIGADAMS, B .

BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1020 (01) :1-24

[7] PEPTIDE AND PROTEIN MOLECULAR-WEIGHT DETERMINATION BY ELECTROPHORESIS USING A HIGH-MOLARITY TRIS BUFFER SYSTEM WITHOUT UREA [J].

FLING, SP ;

GREGERSON, DS .

ANALYTICAL BIOCHEMISTRY, 1986, 155 (01) :83-88

[8]

GAFFRON HANS, 1939, BIOL ZENTRALBL, V59, P302

[9] The chlorophyll-carotenoid proteins of oxygenic photosynthesis [J].

Green, BR ;

Durnford, DG .

ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1996, 47 :685-714

[10] The relation between the photochemical yield and variable fluorescence of photosystem II in the green alga Scenedesmus obliquus [J].

Heinze, I ;

Dau, H ;

Senger, H .

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 1996, 32 (1-2) :89-95