Purification and characterization of an extracellular laccase from the edible mushroom Lentinula edodes, and decolorization of chemically different dyes

被引:222
作者
Nagai, M [1 ]
Sato, T [1 ]
Watanabe, H [1 ]
Saito, K [1 ]
Kawata, M [1 ]
Enei, H [1 ]
机构
[1] Iwate Biotechnol Res Ctr, Iwatsuki, Saitama 0240003, Japan
关键词
D O I
10.1007/s00253-002-1109-2
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A laccase (EC 1.10.3.2) was isolated from the culture filtrate of Lentinula edodes. The enzyme was purified to a homogeneous preparation using hydrophobic, anion-exchange, and size-exclusion chromatographies. SDS-PAGE analysis showed the purified laccase, Lcc 1, to be a monomeric protein of 72.2 kDa. The enzyme had an isoelectric point of around pH 3.0. The optimum pH for enzyme activity was around 4.0, and it was most active at 40degreesC and stable up to 35degreesC. The enzyme contained 23.8% carbohydrate and some copper atoms. The enzyme oxidized 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, p-phenylendiamine, pyrogallol, guaiacol, 2,6-dimethoxyphenol, catechol, and ferulic acid, but not veratryl alcohol, tyrosine, and beta-(3,4-dihydroxyphenyl) alanine. The N-terminal amino acid sequence of Lcc 1 showed close homology to the N-terminal sequences determined for laccases from Phlebia radiata, Trametes villosa, and Trametes versi-color, but only low similarity was observed to a previously reported laccase from L. edodes. Lcc 1 was effective in the decolorization of chemically different dyes - Remazole Brilliant Blue R, Bromophenol Blue, methyl red, and Naphtol Blue Black - without any mediators, but the decolorization of two dyes - red poly(vinylamine)sulfonate-anthrapyridone dye and Reactive Orange 16 - did require some redox mediators.
引用
收藏
页码:327 / 335
页数:9
相关论文
共 42 条
[1]   Microbial decolorization of textile-dye-containing effluents: A review [J].
Banat, IM ;
Nigam, P ;
Singh, D ;
Marchant, R .
BIORESOURCE TECHNOLOGY, 1996, 58 (03) :217-227
[2]   LIGNIN OXIDATION BY LACCASE ISOZYMES FROM TRAMETES-VERSICOLOR AND ROLE OF THE MEDIATOR 2,2'-AZINOBIS(3-ETHYLBENZTHIAZOLINE-6-SULFONATE) IN KRAFT LIGNIN DEPOLYMERIZATION [J].
BOURBONNAIS, R ;
PAICE, MG ;
REID, ID ;
LANTHIER, P ;
YAGUCHI, M .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1995, 61 (05) :1876-1880
[3]   History, overview and applications of mediated lignolytic systems, especially laccase-mediator-systems (Lignozym(R)-process) [J].
Call, HP ;
Mucke, I .
JOURNAL OF BIOTECHNOLOGY, 1997, 53 (2-3) :163-202
[4]   PURIFICATION AND CHARACTERIZATION OF A PHENOLOXIDASE (LACCASE) FROM THE LIGNIN-DEGRADING BASIDIOMYCETE PM1 (CECT-2971) [J].
COLL, PM ;
FERNANDEZABALOS, JM ;
VILLANUEVA, JR ;
SANTAMARIA, R ;
PEREZ, P .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (08) :2607-2613
[5]   Substrate specificity of laccase from Lentinus edodes [J].
D'Annibale, A ;
Celletti, D ;
Felici, M ;
DiMattia, E ;
GiovannozziSermanni, G .
ACTA BIOTECHNOLOGICA, 1996, 16 (04) :257-270
[7]   Biochemical and molecular characterization of a laccase from Marasmius quercophilus [J].
Dedeyan, B ;
Klonowska, A ;
Tagger, S ;
Tron, T ;
Iacazio, G ;
Gil, G ;
Le Petit, J .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (03) :925-929
[8]   Production of multiple laccase isoforms by Phanerochaete chrysosporium grown under nutrient sufficiency [J].
Dittmer, JK ;
Patel, NJ ;
Dhawale, SW ;
Dhawale, SS .
FEMS MICROBIOLOGY LETTERS, 1997, 149 (01) :65-70
[9]   The ligninolytic system of the white rot fungus Pycnoporus cinnabarinus: Purification and characterization of the laccase [J].
Eggert, C ;
Temp, U ;
Eriksson, KEL .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (04) :1151-1158
[10]   BIODEGRADATION OF POLYCYCLIC AROMATIC-HYDROCARBONS BY NEW ISOLATES OF WHITE ROT FUNGI [J].
FIELD, JA ;
DEJONG, E ;
COSTA, GF ;
DEBONT, JAM .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (07) :2219-2226