Plasmodium falciparum:: A novel method for analyzing haplotypes in mixed infections

被引：7

作者：

Certain, Laura K. ^{[1
]}

Sibley, Carol H. ^{[1
]}

机构：

[1] Univ Washington, Dept Genome Sci, Seattle, WA 98195 USA

来源：

EXPERIMENTAL PARASITOLOGY | 2007年 / 115卷 / 03期

关键词：

malaria; microsatellites; drug resistance; dihydrofolate reductase;

D O I：

10.1016/j.exppara.2006.09.003

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Studying the population genetics of Plasmodium falciparum is necessary for understanding the spread of drug resistance. However, these studies are hampered by the inability to determine haplotypes from patient samples that contain multiple parasite populations. Therefore, we have developed a method for separating for genetic analysis the individual strains in a mixed infection. We amplified a 6 kb region of chromosome 4, including the dihydrofolate reductase gene and upstream microsatellite markers. This PCR product was inserted by recombination into a gapped yeast shuttle plasmid containing both selectable and counter-select able markers. Because each plasmid contains only one insert and each yeast colony contains only one plasmid, the individual strains are now separate. We analyzed mixtures of 3D7, K1, and Dd2 DNA and correctly identified a haplotype in each case. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：233 / 241

页数：9

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