Surface expression of Kv1 channels is governed by a C-terminal motif

被引:93
作者
Li, DQ [1 ]
Takimoto, K [1 ]
Levitan, ES [1 ]
机构
[1] Univ Pittsburgh, Dept Pharmacol, Pittsburgh, PA 15261 USA
关键词
D O I
10.1074/jbc.275.16.11597
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Voltage-gated K+ channel subunits must reach the plasma membrane to repolarize action potentials. Yet the efficiency of cell surface targeting varies among Kv subunits with some requiring auxiliary subunits for optimal expression. Here we identify a conserved motif located in the variable C-terminal region of Kv1 channels that controls the efficiency of functional channel expression. Variations among wild type channels in the optimal sequence VXYSL produce differences in distribution and the requirement for auxiliary subunits. Furthermore, deletion of this motif decreases subunit glycosylation and surface localization but does not prohibit subunit multimerization. Finally, the action of the essential sequence is shown to be independent of the chaperone effect of Kv beta subunits. Thus, the newly identified C-terminal motif governs processing and cell surface expression of Kv1 voltage-gated K+ channels.
引用
收藏
页码:11597 / 11602
页数:6
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