Bacteriophage Therapy of Salmonella enterica: A Fresh Appraisal of Bacteriophage Therapy

被引：98

作者：

Capparelli, Rosanna ^{[2
]}

Nocerino, Nunzia ^{[1
]}

Iannaccone, Marco ^{[2
]}

Ercolini, Danilo ^{[2
]}

Parlato, Marianna ^{[2
]}

Chiara, Medaglia ^{[2
]}

Iannelli, Domenico ^{[1
]}

机构：

[1] Univ Naples Federico II, Chair Immunol, I-80055 Naples, Italy

[2] Univ Naples Federico II, Fac Biotechnol, I-80055 Naples, Italy

来源：

JOURNAL OF INFECTIOUS DISEASES | 2010年 / 201卷 / 01期

关键词：

STAPHYLOCOCCUS-AUREUS; SEROVAR ENTERITIDIS; REDUCE SALMONELLA; VIBRIO-CHOLERAE; PHAGE; RESISTANCE; ORIGINS; COST;

D O I：

10.1086/648478

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Background. The most serious criticisms leveled at bacteriophage therapy are as follows: phages induce neutralizing antibodies, phages are active only when administered shortly after bacterial infection, and phage-resistant bacteria emerge rapidly in the course of therapy. Methods. Phages lytic for several Salmonella enterica serovars were isolated by means of standard protocols from feces of patients with gastroenteritis. Growth of S. enterica serovar Paratyphi B (Salp572(phi 1S)) in the presence of phage phi 1 (selected from among 8 phages for its larger host range) provided a phage phi 1 resistant bacterial strain (Salp572(phi 1R)). The properties of the Salp572(phi 1S) and Salp572(phi 1R) strains and of phage phi 1 were studied in a mouse model of experimental infection. Results. Phages induced nonneutralizing antibodies and were active 2 weeks after experimental infection of mice; phage-resistant bacteria were avirulent and short lived in vivo. More importantly, phage-resistant bacteria were excellent vaccines, protecting against lethal doses of heterologous S. enterica serovars. Conclusions. Phage therapy effectiveness has not yet been properly assessed.

引用

页码：52 / 61

页数：10

共 43 条

[1]

Ackermann H W, 1978, Adv Virus Res, V23, P1, DOI 10.1016/S0065-3527(08)60096-2

[2] BACTERIOPHAGE-TYPING DESIGNATIONS OF SALMONELLA-TYPHIMURIUM [J].

ANDERSON, ES ;

WARD, LR ;

DESAXE, MJ ;

DESA, JDH .

JOURNAL OF HYGIENE, 1977, 78 (02) :297-300

[3] Origins and consequences of antimicrobial-resistant nontyphoidal Salmonella:: Implications for the use of fluoroquinolones in food animals [J].

Angulo, FJ ;

Johnson, KR ;

Tauxe, RV ;

Cohen, ML .

MICROBIAL DRUG RESISTANCE, 2000, 6 (01) :77-83

[4] Bacteriophage therapy to reduce Salmonella colonization of broiler chickens [J].

Atterbury, R. J. ;

Van Bergen, M. A. P. ;

Ortiz, F. ;

Lovell, M. A. ;

Harris, J. A. ;

De Boer, A. ;

Wagenaar, J. A. ;

Allen, V. M. ;

Barrow, P. A. .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2007, 73 (14) :4543-4549

[5] Correlation of conversion of Salmonella enterica serovar enteritidis phage type 1, 4, or 6 to phage type 7 with loss of lipopolysaccharide [J].

Baggesen, DL ;

Wegener, HC ;

Madsen, M .

JOURNAL OF CLINICAL MICROBIOLOGY, 1997, 35 (01) :330-333

[6] THE ACTIVITY IN THE CHICKEN ALIMENTARY-TRACT OF BACTERIOPHAGES LYTIC FOR SALMONELLA-TYPHIMURIUM [J].

BERCHIERI, A ;

LOVELL, MA ;

BARROW, PA .

RESEARCH IN MICROBIOLOGY, 1991, 142 (05) :541-549

[7] Linking genetic change to community evolution: insights from studies of bacteria and bacteriophage [J].

Bohannan, BJM ;

Lenski, RE .

ECOLOGY LETTERS, 2000, 3 (04) :362-377

[8] Dynamics of success and failure in phage and antibiotic therapy in experimental infections [J].

Bull, J. J. ;

Levin, Bruce R. ;

DeRouin, Terry ;

Walker, Nina ;

Bloch, Craig A. .

BMC MICROBIOLOGY, 2002, 2 (1) :1-10

[9] Quantitative Models of In Vitro Bacteriophage-Host Dynamics and Their Application to Phage Therapy [J].

Cairns, Benjamin J. ;

Timms, Andrew R. ;

Jansen, Vincent A. A. ;

Connerton, Ian F. ;

Payne, Robert J. H. .

PLOS PATHOGENS, 2009, 5 (01)

[10] Experimental phage therapy against Staphylococcus aureus in mice [J].

Capparelli, Rosanna ;

Parlato, Marianna ;

Borriello, Giorgia ;

Salvatore, Paola ;

Iannelli, Domenico .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2007, 51 (08) :2765-2773

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