Hands-free sample preparation platform for nucleic acid analysis

被引：20

作者：

Baier, T. ^{[1
]}

Hansen-Hagge, T. E. ^{[1
]}

Gransee, R. ^{[1
]}

Crombe, A. ^{[1
]}

Schmahl, S. ^{[1
]}

Paulus, C. ^{[1
]}

Drese, K. S. ^{[1
]}

Keegan, H. ^{[2
]}

Martin, C. ^{[2
,3
]}

O'Leary, J. J. ^{[2
,3
]}

Furuberg, L. ^{[4
]}

Solli, L. ^{[5
]}

Gronn, P. ^{[5
]}

Falang, I. M. ^{[5
]}

Karlgard, A. ^{[5
]}

Gulliksen, A. ^{[5
]}

Karlsen, F. ^{[5
]}

机构：

[1] Inst Mikrotech Mainz, D-55129 Mainz, Germany

[2] Coombe Women & Infants Univ Hosp, Dept Pathol, Dublin 8, Ireland

[3] Univ Dublin Trinity Coll, Dept Histopathol, Dublin 2, Ireland

[4] MiNaLab Facil, SINTEF ICT, N-0373 Oslo, Norway

[5] NorChip AS, N-3490 Klokkarstua, Norway

来源：

LAB ON A CHIP | 2009年 / 9卷 / 23期

关键词：

DNA EXTRACTION; MESSENGER-RNA; HPV-PROOFER; PURIFICATION; MICROCHIP; PCR;

D O I：

10.1039/b910421f

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A Lab-On-Chip system with an instrument is presented which is capable of performing total sample preparation and automated extraction of nucleic acid from human cell samples fixed in a methanol based solution. The target application is extraction of mRNA from cervical liquid based cytology specimens for detection of transformed HPV-infections. The device accepts 3 ml of sample and performs the extraction in a disposable polymer chip of credit card size. All necessary reagents for cell lysis, washing, and elution are stored on-chip and the extraction is performed in two filter stages; one for cell pre-concentration and the other for nucleic acid capture. Tests performed using cancer cell lines and cervical liquid based cytology specimens confirm the extraction of HPV-mRNA by the system.

引用

收藏

页码：3399 / 3405

页数：7

相关论文

共 20 条

[1] Polymer microfabrication technologies for microfluidic systems [J].

Becker, Holger ;

Gaertner, Claudia .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2008, 390 (01) :89-111

[2] Microchip-based cell lysis and DNA extraction from sperm cells for application to forensic analysis [J].

Bienvenue, JM ;

Duncalf, N ;

Marchiarullo, D ;

Ferrance, JP ;

Landers, JP .

JOURNAL OF FORENSIC SCIENCES, 2006, 51 (02) :266-273

[3] RAPID AND SIMPLE METHOD FOR PURIFICATION OF NUCLEIC-ACIDS [J].

BOOM, R ;

SOL, CJA ;

SALIMANS, MMM ;

JANSEN, CL ;

WERTHEIMVANDILLEN, PME ;

VANDERNOORDAA, J .

JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (03) :495-503

[4] One-step pathogen specific DNA extraction from whole blood on a centrifugal microfluidic device [J].

Cho, Yoon-Kyoung ;

Lee, Jeong-Gun ;

Park, Jong-Myeon ;

Lee, Beom-Seok ;

Lee, Youngsun ;

Ko, Christopher .

LAB ON A CHIP, 2007, 7 (05) :565-573

[5] Integrated microfluidic tmRNA purification and real-time NASBA device for molecular diagnostics [J].

Dimov, Ivan K. ;

Garcia-Cordero, Jose L. ;

O'Grady, Justin ;

Poulsen, Claus R. ;

Viguier, Caroline ;

Kent, Lorcan ;

Daly, Paul ;

Lincoln, Bryan ;

Maher, Majella ;

O'Kennedy, Richard ;

Smith, Terry J. ;

Ricco, Antonio J. ;

Lee, Luke P. .

LAB ON A CHIP, 2008, 8 (12) :2071-2078

[6] A fully integrated microfluidic genetic analysis system with sample-in-answer-out capability [J].

Easley, Christopher J. ;

Karlinsey, James M. ;

Bienvenue, Joan M. ;

Legendre, Lindsay A. ;

Roper, Michael G. ;

Feldman, Sanford H. ;

Hughes, Molly A. ;

Hewlett, Erik L. ;

Merkel, Tod J. ;

Ferrance, Jerome P. ;

Landers, James P. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (51) :19272-19277

[7]

Gulliksen A, 2005, LAB CHIP, V5, P416, DOI 10.1039/b415525d

[8] Microfluidic platforms for lab-on-a-chip applications [J].

Haeberle, Stefan ;

Zengerle, Roland .

LAB ON A CHIP, 2007, 7 (09) :1094-1110

[9] A nanoliter-scale nucleic acid processor with parallel architecture [J].

Hong, JW ;

Studer, V ;

Hang, G ;

Anderson, WF ;

Quake, SR .

NATURE BIOTECHNOLOGY, 2004, 22 (04) :435-439

[10] Presence of E6 and E7 mRNA from human papillomavirus types 16, 18, 31, 33, and 45 in the majority of cervical carcinomas [J].

Kraus, I ;

Molden, T ;

Holm, R ;

Lie, AK ;

Karlsen, F ;

Kristensen, GB ;

Skomedal, H .

JOURNAL OF CLINICAL MICROBIOLOGY, 2006, 44 (04) :1310-1317