GABAergic neurotransmission in rat taste buds: immunocytochemical study for GABA and GABA transporter subtypes

被引:42
作者
Obata, H
Shimada, K
Sakai, N
Saito, N
机构
[1] KOBE UNIV,BIOSIGNAL RES CTR,MOL PHARMACOL LAB,NADA KU,KOBE,HYOGO 657,JAPAN
[2] KOBE UNIV,SCH MED,DEPT MAXILLOFACIAL SURG,KOBE,HYOGO 650,JAPAN
来源
MOLECULAR BRAIN RESEARCH | 1997年 / 49卷 / 1-2期
关键词
gamma-Aminobutyric acid (GABA); transporter; taste bud; immunocytochemistry; gustatory; tongue;
D O I
10.1016/S0169-328X(97)00118-6
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
gamma-Aminobutyric acid (GABA) is known to be a candidate for the neurotransmitter involved in the sense of taste. We hereby studied GABA and its termination system, GABA transporters, in rat taste buds by immunocytochemical approaches. Immunoblot analysis of three GABA transporter subtypes (GAT1, GAT2 and GAT3) revealed that the immunoreactive bands of GAT2 and GAT3, but not GAT1, were detected in the tongue. GAT3-immunoreactive band was recognized only in the circumvallatepapilla containing a large number of taste buds while GAT2-immunoreactive bands were seen in all areas of the tongue. GAT2 immunoreactivity appeared to be specifically in the nerve fibers beneath the lingual epithelium. Both GAT3 and GABA immunoreactivities were detected only in taste buds. A few GAT3-immunoreactive cells were found in a cross-section of each taste bud but most GAT3-immunoreactive cells were localized in the margin of the taste bud. GAT3 was predominantly concentrated in the distal portion of the GAT3-immunoreactive cells. In contrast, GABA-immunoreactive cells were seen more frequently within each taste bud and the immunoreactivity was distributed throughout the perikarya of the cells. These results suggest that the GABA-uptake system is present in the taste buds and the GABAergic neurotransmission involved in the sensation of taste is terminated by the uptake of GABA into certain taste cells via GAT3. (C) 1997 Elsevier Science B.V.
引用
收藏
页码:29 / 36
页数:8
相关论文
共 36 条
[1]  
BORDEN LA, 1992, J BIOL CHEM, V267, P21105
[2]   FUNCTIONAL EXPRESSION AND CNS DISTRIBUTION OF A BETA-ALANINE-SENSITIVE NEURONAL GABA TRANSPORTER [J].
CLARK, JA ;
DEUTCH, AY ;
GALLIPOLI, PZ ;
AMARA, SG .
NEURON, 1992, 9 (02) :337-348
[3]  
DELAY RJ, 1994, J NEUROSCI, V14, P6132
[4]   MERKEL-LIKE BASAL CELLS IN NECTURUS TASTE-BUDS CONTAIN SEROTONIN [J].
DELAY, RJ ;
TAYLOR, R ;
ROPER, SD .
JOURNAL OF COMPARATIVE NEUROLOGY, 1993, 335 (04) :606-613
[5]   ULTRASTRUCTURE OF TASTE CELLS AND SYNAPSES IN THE MUDPUPPY NECTURUS-MACULOSUS [J].
DELAY, RJ ;
ROPER, SD .
JOURNAL OF COMPARATIVE NEUROLOGY, 1988, 277 (02) :268-280
[6]  
EWALD DA, 1994, J NEUROSCI, V14, P3791
[7]   CLONING AND EXPRESSION OF A RAT-BRAIN GABA TRANSPORTER [J].
GUASTELLA, J ;
NELSON, N ;
NELSON, H ;
CZYZYK, L ;
KEYNAN, S ;
MIEDEL, MC ;
DAVIDSON, N ;
LESTER, HA ;
KANNER, BI .
SCIENCE, 1990, 249 (4974) :1303-1306
[8]   IMMUNOCYTOCHEMICAL LOCALIZATION OF 3 SUBTYPES OF GABA TRANSPORTER IN RAT RETINA [J].
HONDA, S ;
YAMAMOTO, M ;
SAITO, N .
MOLECULAR BRAIN RESEARCH, 1995, 33 (02) :319-325
[9]   PRODUCTION OF SPECIFIC ANTIBODIES AGAINST GABA-TRANSPORTER SUBTYPES (GAT1, GAT2, GAT3) AND THEIR APPLICATION TO IMMUNOCYTOCHEMISTRY [J].
IKEGAKI, N ;
SAITO, N ;
HASHIMA, M ;
TANAKA, C .
MOLECULAR BRAIN RESEARCH, 1994, 26 (1-2) :47-54
[10]  
Itouji A, 1996, MOL BRAIN RES, V37, P309