In vitro and in vivo stability of new multilamellar vesicles

被引：37

作者：

Freund, O

Amédee, J

Roux, D

Laversanne, R

机构：

[1] Lab LEMI, F-33650 Martillac, France

[2] Univ Victor Segalen, INSERM U443, F-33076 Bordeaux, France

[3] CAPSULIS SA, F-33600 Pessac, France

[4] Ctr Rech Paul Pascal, CNRS, F-3600 Pessac, France

来源：

LIFE SCIENCES | 2000年 / 67卷 / 04期

关键词：

multilamellar vesicles; protein encapsulation; spherulite; oral administration; gastrointestinal tract; blood stream;

D O I：

10.1016/S0024-3205(00)00640-8

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Factors affecting multilamellar vesicles transport to the blood compartment after oral administration to rats were evaluated first in vitro. A high entrapment of protein A was obtained when the vesicles were prepared by shearing a lyotropic lamellar phase composed of soybean phosphatidylcholine, cholesterol and polyoxyethylene alcohol (C12H25(OCH2CH2)(4)OH) as neutral detergent. In vitro tests showed that these vesicles (spherulites(R)) were stabled in 50% of fetal calf serum, in acidic (pH 3) or basic (pH 10) buffers, in pancreatin but are partially lysed in 20mM bile salts. Oral administration of spherulites entrapping In-111-NTA in fasting rats showed a increase of radioacticivity in blood. This could be explained by passage of some spherulites in the enterocytes. (C) 2000 Elsevier Science Inc. All rights reserved.

引用

页码：411 / 419

页数：9

共 16 条

[1] Encapsulation of calcitonin in liposomes depends on the vesicle preparation method [J].