DNA-WT1 protein interaction studied by surface-enhanced Raman spectroscopy

被引：7

作者：

Joshi, Bhuwan ^{[1
]}

Chakrabarty, Ayan ^{[1
]}

Bruot, Christopher ^{[1
]}

Ainsworth, Hannah ^{[1
]}

Fraizer, Gail ^{[2
]}

Wei, Qi-Huo ^{[1
]}

机构：

[1] Kent State Univ, Inst Liquid Crystal, Kent, OH 44242 USA

[2] Kent State Univ, Dept Biol Sci, Kent, OH 44242 USA

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2010年 / 396卷 / 04期

关键词：

SERS; Protein-DNA interactions; Surface plasmons; WT1; TUMOR-SUPPRESSOR; BINDING-SITES; DNA; SCATTERING; WT1; NANOPARTICLES; MOLECULES; GROWTH; SERRS; ASSAY;

D O I：

10.1007/s00216-009-3364-x

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

Interactions of proteins with DNA play an important role in regulating the biological functions of DNA. Here we propose and demonstrate the detection of protein-DNA binding using surface-enhanced Raman scattering (SERS). In this method, double-stranded DNA molecules with potential protein-binding sites are labeled with dye molecules and immobilized on metal nanoparticles. The binding of proteins protects the DNA from complete digestion by exonuclease and can be detected by measuring the SERS signals before and after the exonuclease digestion. As a proof of concept, this SERS-based protein-DNA interaction assay is validated by studying the binding of a zinc finger transcription factor WT1 with DNA sequences derived from the promoter of the human vascular endothelial growth factor.

引用

页码：1415 / 1421

页数：7

共 45 条

[1]

Detection of sequence-specific protein-DNA interactions via surface enhanced resonance Raman scattering [J].