Characterization of the Atg17-Atg29-Atg31 complex specifically required for starvation-induced autophagy in Saccharomyces cerevisiae

被引：103

作者：

Kabeya, Yukiko ^{[1
]}

Noda, Nobuo N. ^{[2
]}

Fujioka, Yuko ^{[2
]}

Suzuki, Kuninori ^{[1
]}

Inagaki, Fuyuhiko ^{[2
]}

Ohsumi, Yoshinori ^{[1
]}

机构：

[1] Tokyo Inst Technol, Integrated Res Inst, Adv Res Org, Midori Ku, Yokohama, Kanagawa 2268503, Japan

[2] Hokkaido Univ, Grad Sch Pharmaceut Sci, Dept Biol Struct, Kita Ku, Sapporo, Hokkaido 0010021, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 389卷 / 04期

关键词：

Autophagy; Starvation; ATC; Autophagosome; PAS; Rapamycin; YEAST; PROTEIN; ORGANIZATION; GENES; ATG13; CELLS;

D O I：

10.1016/j.bbrc.2009.09.034

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Nutrient starvation induces autophagy to degrade cytoplasmic materials in the vacuole/lysosomes. In the yeast, Saccharomyces cerevisiae, Atg17, Atg29, and Atg31/Cis1 are specifically required for autophagosome formation by acting as a scaffold complex essential for pre-autophagosomal structure (PAS) organization. Here, we show that these proteins constitutively form an Atg17-Atg29-Atg31 ternary complex, in which phosphorylated Atg31 is included. Reconstitution analysis of the ternary complex in E coli indicates that. the three proteins are included in equimolar amounts in the complex. The molecular mass of a monomeric Atg17-Atg29-Atg31 complex is calculated at 97 kDa; however, analytical ultracentrifugation shows that the molecular mass of the ternary complex is 198 kDa, suggesting a dimeric complex. We propose that this ternary complex acts as a functional unit for autophagosome formation. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：612 / 615

页数：4

共 18 条

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