The completion of the Mammalian Gene Collection (MGC)

被引：105

作者：

Temple, Gary ^{[1
]}

Gerhard, Daniela S. ^{[2
]}

Rasooly, Rebekah ^{[3
]}

Feingold, Elise A.

Good, Peter J.

Robinson, Cristen

Mandich, Allison

Derge, Jeffrey G. ^{[4
]}

Lewis, Jeanne ^{[4
]}

Shoaf, Debonny ^{[4
]}

Collins, Francis S.

Jang, Wonhee ^{[5
]}

Wagner, Lukas ^{[5
]}

Shenmen, Carolyn M. ^{[5
]}

Misquitta, Leonie ^{[6
]}

Schaefer, Carl F. ^{[6
]}

Buetow, Kenneth H. ^{[6
]}

Bonner, Tom I. ^{[7
]}

Yankie, Linda ^{[5
]}

Ward, Ming ^{[5
]}

Phan, Lon ^{[5
]}

Astashyn, Alex ^{[5
]}

Brown, Garth ^{[5
]}

Farrell, Catherine ^{[5
]}

Hart, Jennifer ^{[5
]}

Landrum, Melissa ^{[5
]}

Maidak, Bonnie L. ^{[5
]}

Murphy, Michael ^{[5
]}

Murphy, Terence ^{[5
]}

Rajput, Bhanu ^{[5
]}

Riddick, Lillian ^{[5
]}

Webb, David ^{[5
]}

Weber, Janet ^{[5
]}

Wu, Wendy ^{[5
]}

Pruitt, Kim D. ^{[5
]}

Maglott, Donna ^{[5
]}

Siepel, Adam ^{[8
]}

Brejova, Brona ^{[8
]}

Diekhans, Mark ^{[9
]}

Harte, Rachel ^{[9
]}

Baertsch, Robert ^{[9
]}

Kent, Jim ^{[9
]}

Haussler, David ^{[9
]}

Brent, Michael ^{[10
,11
]}

Langton, Laura ^{[10
]}

Comstock, Charles L. G. ^{[10
]}

Stevens, Michael ^{[11
]}

Wei, Chaochun ^{[10
]}

van Baren, Marijke J. ^{[10
]}

Salehi-Ashtiani, Kourosh ^{[12
,13
]}

机构：

[1] NHGRI, NIH, Genome Technol Branch, Bethesda, MD 20892 USA

[2] NCI, NIH, Bethesda, MD 20892 USA

[3] NIDDK, NIH, Bethesda, MD 20892 USA

[4] NCI, SAIC Frederic Inc, Frederick, MD 21702 USA

[5] Natl Lib Med, Natl Ctr Biotechnol Informat, Bethesda, MD 20894 USA

[6] NCI, Ctr Bioinformat, Rockville, MD 20852 USA

[7] NIMH, NIH, Bethesda, MD 20892 USA

[8] Cornell Univ, Ithaca, NY 14853 USA

[9] Univ Calif Santa Cruz, Ctr Biomol Sci & Engn, Santa Cruz, CA 95064 USA

[10] Washington Univ, Ctr Genome Sci, St Louis, MO 63130 USA

[11] Washington Univ, Dept Comp Sci, St Louis, MO 63130 USA

[12] Dana Farber Canc Inst, Ctr Canc Syst Biol, Boston, MA 02115 USA

[13] Dana Farber Canc Inst, Dept Canc Biol, Boston, MA 02115 USA

[14] Lawrence Livermore Natl Lab, IMAGE Consortium, Biol & Biotechnol Res Program, Livermore, CA 94550 USA

[15] Open Biosyst, Huntsville, AL 35806 USA

[16] HudsonAlpha Inst Biotechnol, Huntsville, AL 35806 USA

[17] Med Coll Wisconsin, Dept Dermatol, Milwaukee, WI 53226 USA

[18] Express Genom Inc, Frederick, MD 21701 USA

[19] Baylor Coll Med, Human Genome Sequencing Ctr, Houston, TX 77030 USA

[20] Univ Houston, Houston, TX 77004 USA

[21] Natl Human Genome Inst, NIH Intramural Sequencing Ctr, NIH, Bethesda, MD 20892 USA

[22] Stanford Univ, Dept Genet, Stanford Human Genome Ctr, Sch Med, Stanford, CA 94305 USA

[23] BC Canc Agcy, Genome Sci Ctr, Vancouver, BC V5Z 4S6, Canada

[24] GeneCopoeia Inc, Rockville, MD 20850 USA

[25] Beckman Coulter Genom, Beverly, MA 01915 USA

[26] Geneart AG, D-93053 Regensburg, Germany

[27] Codon Devices Inc, Cambridge, MA 02139 USA

[28] NCI SAIC Frederick, Prot Express Lab, Frederick, MD 21702 USA

[29] Kazusa DNA Res Inst, Chiba 2920818, Japan

[30] German Canc Res Ctr, D-69120 Heidelberg, Germany

来源：

GENOME RESEARCH | 2009年 / 19卷 / 12期

基金：

美国国家卫生研究院;

关键词：

GENOME BROWSER DATABASE; EDITING SITES; RNA; IDENTIFICATION; RECOVERY; SEQUENCE; IMPROVE;

D O I：

10.1101/gr.095976.109

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Since its start, the Mammalian Gene Collection (MGC) has sought to provide at least one full-protein-coding sequence cDNA clone for every human and mouse gene with a RefSeq transcript, and at least 6200 rat genes. The MGC cloning effort initially relied on random expressed sequence tag screening of cDNA libraries. Here, we summarize our recent progress using directed RT-PCR cloning and DNA synthesis. The MGC now contains clones with the entire protein-coding sequence for 92% of human and 89% of mouse genes with curated RefSeq (NM-accession) transcripts, and for 97% of human and 96% of mouse genes with curated RefSeq transcripts that have one or more PubMed publications, in addition to clones for more than 6300 rat genes. These high-quality MGC clones and their sequences are accessible without restriction to researchers worldwide.

引用

页码：2324 / 2333

页数：10

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