Detection of potentially pathogenic bacteria in the drinking water distribution system of a hospital in Hungary

被引:56
作者
Felfoldi, T. [1 ]
Heeger, Z. [1 ]
Vargha, M. [2 ,3 ]
Marialigeti, K. [1 ]
机构
[1] Eotvos Lorand Univ, Dept Microbiol, Fac Sci, H-1117 Budapest, Hungary
[2] Natl Inst Environm Hlth, Dept Water Hyg, Budapest, Hungary
[3] Natl Inst Environm Hlth, Dept Water Hyg, Budapest, Hungary
关键词
Cloning and sequencing; Legionella; PCR detection; Pseudomonas aeruginosa; waterborne nosocomial infections; POLYMERASE-CHAIN-REACTION; RIBOSOMAL-RNA SEQUENCE; LEGIONELLA-PNEUMOPHILA; PSEUDOMONAS-AERUGINOSA; CYSTIC-FIBROSIS; PCR; DNA; CULTURE;
D O I
10.1111/j.1469-0691.2009.02795.x
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
The drinking water distribution system of a hospital was investigated using standard cultivation techniques, taxon-specific PCRs targeting pathogenic bacteria, denaturing gradient gel electrophoresis, cloning and sequencing. The results obtained verify the higher sensitivity of PCR compared to cultivation for detecting Legionella and Pseudomonas aeruginosa. Moreover, several other opportunistic pathogenic bacteria, such as Escherichia albertii, Acinetobacter lwoffi and Corynebacterium tuberculostrearicum, were detected, emphasizing that drinking water systems, especially those with stagnant water sections, could be the source of nosocomial infections.
引用
收藏
页码:89 / 92
页数:4
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