Neutrophil chemotaxis and superoxide production are induced by cross-linking FcγRII receptors

Neutrophils express two types of receptor for the Fc region of IgG, Fc gamma RII and Fc gamma RIIIB. Via these receptors, neutrophils bind IgG complexes that contain more than one IgG molecule. This binding activates functional processes, such as the respiratory burst and chemotaxis. Neutrophils were treated with biotinylated anti-Fc receptor monoclonal antibodies and chemotaxis toward streptavidin, a cross-linking agent, was determined. Cross-linking Fc gamma RII and not Fc gamma RIIIB induced neutrophil chemotaxis. Superoxide production in response to immobilized anti-Fc receptor antibodies was also examined. Anti-Fc gamma RII Fab bound to ELISA plates induced superoxide production, while anti-Fc gamma RIIIB Fab did not. Pretreatment of neutrophils with anti-Fc gamma RII Fab reduced superoxide generated by immobilized anti-Fc gamma RII antibody. The data demonstrate that Fc gamma RII and not Fc gamma RIIIB are responsible for neutrophil chemotaxis and superoxide production upon Fc receptor activation, (C) 2000 Academic Press.