Tackling the phosphoproteome: tools and strategies

被引：162

作者：

Kalume, DE

Molina, H

Pandey, A

机构：

[1] Johns Hopkins Univ, McKusick Nathans Inst Genet Med, Baltimore, MD 21287 USA

[2] Johns Hopkins Univ, Dept Biol Chem, Baltimore, MD 21287 USA

来源：

CURRENT OPINION IN CHEMICAL BIOLOGY | 2003年 / 7卷 / 01期

关键词：

D O I：

10.1016/S1367-5931(02)00009-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Characterization of post-translational modifications in proteins is one of the major tasks that is to be accomplished in the post-genomic era. Phosphorylation is a key reversible modification that regulates enzymatic activity, subcellular localization, complex formation and degradation of proteins. Rapid advances in mass spectrometry instrumentation coupled to the development of analytical methods over the past several years now allow us to investigate the phosphoproteome on a global scale.

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页码：64 / 69

页数：6

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