Exendin-4, a GLP-1 receptor agonist, directly induces adiponectin expression through protein kinase A pathway and prevents inflammatory adipokine expression

被引：168

作者：

Chung, Le Thi Kim ^{[1
]}

Hosaka, Toshio ^{[2
]}

Yoshida, Masaki ^{[1
]}

Harada, Nagakatsu ^{[1
]}

Sakaue, Hiroshi ^{[1
]}

Sakai, Tohru ^{[2
]}

Nakaya, Yutaka ^{[1
]}

机构：

[1] Univ Tokushima, Grad Sch, Inst Hlth Biosci, Dept Nutr & Metab, Tokushima 7708503, Japan

[2] Univ Tokushima, Grad Sch, Inst Hlth Biosci, Dept Publ Hlth & Appl Nutr, Tokushima 7708503, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 390卷 / 03期

关键词：

Exendin-4; Adiponectin; GLP-1; GLUCAGON-LIKE PEPTIDE-1; PANCREATIC BETA-CELL; TUMOR-NECROSIS-FACTOR; INSULIN-RESISTANCE; 3T3-L1; ADIPOCYTES; SIGNAL-TRANSDUCTION; GLUCOSE-TRANSPORT; GENE-EXPRESSION; ADIPOSE-TISSUE; INTERLEUKIN-6;

D O I：

10.1016/j.bbrc.2009.10.015

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Exendin-4 (Ex-4) is a glucagon-like peptide-1 receptor (GLP-1R) agonist that has been used as a drug injected subcutaneously for treatment of type 2 diabetes. Many studies have revealed molecular targets of Ex-4, but its influence on adipokines has not been determined. Our study showed that Ex-4 induced secretion of adiponectin into the culture medium of 3T3-L1 adipocytes. This effect of Ex-4 is due to increased adiponectin mRNA level through the GLP-1R. Both forskolin and 3-isobutyl-1-methylxanthine (IBMX), which may finally elevate cyclic adenosine monophosphate (cAMP) concentration, prevented the induction of adiponectin expression by Ex-4. Moreover, H89, a protein kinase A inhibitor, blocked the effect of Ex-4 on adiponectin. On the other hand, Ex-4 decreased the mRNA levels of inflammatory adipokines. The results indicate that Ex-4 directly promotes adiponectin secretion via the protein kinase A pathway in 3T3-L1 adipocytes and may ameliorate insulin resistance. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：613 / 618

页数：6

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