Arabidopsis boron transporter for xylem loading

被引：479

作者：

Takano, J ^{[1
]}

Noguchi, K ^{[1
]}

Yasumori, M ^{[1
]}

Kobayashi, M ^{[1
]}

Gajdos, Z ^{[1
]}

Miwa, K ^{[1
]}

Hayashi, H ^{[1
]}

Yoneyama, T ^{[1
]}

Fujiwara, T ^{[1
]}

机构：

[1] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Appl Biol Chem, Bunkyo Ku, Tokyo 1138657, Japan

来源：

NATURE | 2002年 / 420卷 / 6913期

关键词：

D O I：

10.1038/nature01139

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Boron deficiency hampers the productivity of 132 crops in more than 80 countries(1). Boron is essential in higher plants primarily for maintaining the integrity of cell walls(2-4) and is also beneficial and might be essential in animals(5) and in yeast(6). Understanding the molecular mechanism(s) of boron transport is crucial for alleviating boron deficiency. Here we describe the molecular identification of boron transporters in biological systems. The Arabidopsis thaliana mutant bor1-1 is sensitive to boron deficiency(7,8). Uptake studies indicated that xylem loading is the key step for boron accumulation in shoots with a low external boron supply and that the bor1-1 mutant is defective in this process. Positional cloning identified BOR1 as a membrane protein with homology to bicarbonate transporters in animals. Moreover, a fusion protein of BOR1 and green fluorescent protein (GFP) localized to the plasma membrane in transformed cells. The promoter of BOR1 drove GFP expression in root pericycle cells. When expressed in yeast, BOR1 decreased boron concentrations in cells. We show here that BOR1 is an efflux-type boron transporter for xylem loading and is essential for protecting shoots from boron deficiency.

引用

页码：337 / 340

页数：4

共 30 条

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