Characteristics of scrapie isolates derived from hay mites

被引:25
作者
Carp, RI
Meeker, HC
Rubenstein, R
Sigurdarson, S
Papini, M
Kascsak, RJ
Kozlowski, PB
Wisniewski, HM
机构
[1] New York State Inst Basic Res Dev Disabil, Dept Virol, Staten Isl, NY 10314 USA
[2] New York State Inst Basic Res Dev Disabil, Dept Neurobiol, Staten Isl, NY 10314 USA
[3] Univ Iceland, Dept Vet Diagnost, IS-112 Reykjavik, Iceland
关键词
scrapie; hay mites; primary isolates; genetic markers; incubation period;
D O I
10.3109/13550280009013157
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Previous epidemiological evidence suggested that in some instances a vector and/or reservoir is involved in the occurrence and spread of transmissible spongiform encephalopathies (TSEs). In a preliminary study, hay mite preparations from five Icelandic farms with a history of scrapie were injected into mice, and some of these mice became sick after long incubation periods. To confirm that the disease was scrapie, subsequent passages in mice were performed. In addition, the characteristics of the disease process in these passages were assessed and the results compared to those findings with standard scrapie strains. As expected for scrapie, subsequent passages in the same host led to shortened incubation periods compared to those in primary isolate mice, and all mice had spongiform changes in brain. Results were similar for three of four isolates with regard to clinical manifestations, the incubation periods in mice of the three scrapie incubation-period genotypes (s7s7, s7p7, p7p7), and the PrPSc Western blot (WB) pattern. The characteristics of the fourth isolate were markedly different from the other three isolates with regard to these parameters. Comparison of the characteristics of standard mouse-adapted scrapie strains and the four isolates revealed differences; these differences were particularly pronounced for the fourth isolate.
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页码:137 / 144
页数:8
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