Effects of sinomenine on proliferation and apoptosis in human lung cancer cell line NCI-H460 in vitro

被引：79

作者：

Jiang, Tingshu ^{[1
]}

Zhou, Liping ^{[2
]}

Zhang, Wenlu ^{[3
]}

Qu, Dan ^{[1
]}

Xu, Xiaoman ^{[1
]}

Yang, Ying ^{[4
]}

Li, Shengqi ^{[1
]}

机构：

[1] China Med Univ, Shengjing Hosp, Dept Resp Med, Shenyang 110001, Liaoning, Peoples R China

[2] China Med Univ, Hosp 1, Dept Lab Med, Shenyang 110001, Liaoning, Peoples R China

[3] China Med Univ, Hosp 1, Dept Med Cardiol, Shenyang 110001, Liaoning, Peoples R China

[4] Liaoning Med Univ, Affiliated Hosp 1, Dept Oncol, Shenyang 110001, Liaoning, Peoples R China

来源：

MOLECULAR MEDICINE REPORTS | 2010年 / 3卷 / 01期

关键词：

sinomenine; apoptosis; NCI-H460 lung cancer cell line; BCL-2; FAMILY; REQUIREMENT; ACTIVATION; THERAPY;

D O I：

10.3892/mmr_00000217

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

Sinomenine (SIN) is an herbal ingredient isolated from Sinomenium acutum Rehd. et Wils. We evaluated the effects of SIN on the growth and apoptosis of human lung cancer NCI-H460 cells. SIN inhibited the proliferation of the cells in a dose- and time-dependent manner. Apoptosis was confirmed by flow cytometry, the TUNEL assay and transmission electron microscopy. SIN-induced apoptosis was accompanied by the collapse of mitochondrial membrane potential, the release of cytochrome C and the activation of caspase-9 and -3. SIN also increased Bax and decreased Bcl-2 protein levels in the NCI-H460 cells. Data analysis suggested that SIN exerted a significant apoptotic effect oil NCI-H460 cells through the mitochondrial pathway. SIN is therefore a promising chemopreventive candidate for lung carcinoma.

引用

页码：51 / 56

页数：6

共 26 条

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