Short, highly cross-linked, polymer based, monolithic column for capillary electrochromatography

Capillary electrochromatography with a short and highly crosslinked butyl methacrylate based negative charged monolith as stationary phase was developed. The reproducibility of the column in different kinds of mobile phase was investigated. Moreover, the effects of the operational parameters such as, applied voltage, salt concentration, pH, and organic modifier content in the mobile phase on EOF, as well as the retention mechanism of small neutral compounds on such a column were studied systematically. It was found that the reproducibility and stability of the column is satisfactory in the buffer with very extreme pH values. The retention mechanism of neutral solutes on such a column proved to be similar to that of reversed phase high performance liquid chromatography.