Formaldehyde-Inactivated Whole-Virus Vaccine Protects a Murine Model of Enterovirus 71 Encephalomyelitis against Disease

被引：112

作者：

Ong, Kien Chai ^{[2
]}

Devi, Shamala ^{[3
]}

Cardosa, Mary Jane ^{[4
]}

Wong, Kum Thong ^{[1
]}

机构：

[1] Univ Malaya, Fac Med, Dept Pathol, Kuala Lumpur 50603, Malaysia

[2] Univ Malaya, Fac Med, Dept Mol Med, Kuala Lumpur 50603, Malaysia

[3] Univ Malaya, Fac Med, Dept Med Microbiol, Kuala Lumpur 50603, Malaysia

[4] Univ Malaysia Sarawak, Inst Community Hlth & Med, Kota Samarahan, Sarawak, Malaysia

来源：

JOURNAL OF VIROLOGY | 2010年 / 84卷 / 01期

关键词：

CENTRAL-NERVOUS-SYSTEM; MOUTH-DISEASE; MOLECULAR EPIDEMIOLOGY; TYPE-71; INFECTIONS; HAND; FOOT; MICE; OUTBREAK; MALAYSIA; VP1;

D O I：

10.1128/JVI.00999-09

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Enterovirus 71 (EV71) causes childhood hand, foot, and mouth disease and neurological complications, and no vaccines or therapeutic drugs are currently available. Formaldehyde-inactivated whole-virus vaccines derived from EV71 clinical isolates and a mouse-adapted virus (MAV) were tested in a mouse model of EV71 encephalomyelitis. After only two immunizations, given to mice at 1 and 7 days of age, the MAV vaccine protected mice at 14 days of age from disease. Tissues from immunized mice were negative for virus by viral culture, reverse transcriptase PCR, immunohistochemistry analysis, and in situ hybridization. Cross-neutralizing EV71 antibodies to strains with genotypes B3, B4, and C1 to C5 generated in immunized adult mice were able to passively protect 14-day-old mice from disease.

引用

页码：661 / 665

页数：5

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