The immunologically distinct O antigens from Francisella tularensis subspecies tularensis and Francisella novicida are both virulence determinants and protective antigens

被引：81

作者：

Thomas, Rebecca M. ^{[1
]}

Titball, Richard W.

Oyston, Petra C. F.

Griffin, Kate

Waters, Emma

Hitchen, Paul G.

Michell, Stephen L.

Grice, I. Darren

Wilson, Jennifer C.

Prior, Joann L.

机构：

[1] Def Sci & Technol Lab, Salisbury SP4 0JQ, Wilts, England

[2] Univ London Imperial Coll Sci Technol & Med, Fac Nat Sci, Div Mol Biosci, London SW7 2AZ, England

[3] Griffith Univ, Inst Glycom, Brisbane, Qld 4215, Australia

[4] London Sch Hyg & Trop Med, Dept Infect & Trop Dis, London WC1E 7H7, England

[5] Univ Leicester, Dept Infect Immun & Inflammat, Leicester LE1 7RH, Leics, England

来源：

INFECTION AND IMMUNITY | 2007年 / 75卷 / 01期

关键词：

D O I：

10.1128/IAI.01241-06

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We have determined the sequence of the gene cluster encoding the O antigen in Francisella novicida and compared it to the previously reported O-antigen cluster in Francisella tularensis subsp. tularensis. Immunization with purified lipopolysaccharide (LPS) from F. tularensis subsp. tularensis or F. novicida protected against challenge with Francisella tularensis subsp. holarctica and F. novicida, respectively. The LPS from F. tularensis subsp. tularensis did not confer protection against challenge with F. novicida, and the LPS from F. novicida did not confer protection against challenge with F. tularensis subsp. holarctica. Allelic replacement mutants of F. tularensis subsp. tularensis or F. novicida which failed to produce O antigen were attenuated, but exposure to these mutants did not induce a protective immune response. The O antigen of F. tularensis subsp. tularensis appeared to be important for intracellular survival whereas the O antigen of F. novicida appeared to be critical for serum resistance and less important for intracellular survival.

引用

页码：371 / 378

页数：8

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