Using green fluorescent proteins to stud G-protein-coupled receptor localization and trafficking

被引:100
作者
Kallal, L
Benovic, JL
机构
[1] Thomas Jefferson Univ, Dept Microbiol & Immunol, Kimmel Canc Ctr, Philadelphia, PA 19107 USA
[2] SmithKline Beecham, King Of Prussia, PA 19406 USA
关键词
D O I
10.1016/S0165-6147(00)01477-2
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
G-protein-coupled receptors (GPCRs) mediate a diverse array of biological functions as a result of their ability to respond selectively to extra cellular stimuli, which ultimately results in cell-specific activation of signaling cascades. Generally, GPCR activation is followed rapidly by a loss of responsiveness, termed desensitization, which is then followed by a period of recovery or resensitization, These changes in signaling potential are tightly regulated, primarily via mechanisms that involve GPCR phosphorylation and trafficking to distinct locations within the cell. Tagging of GPCRs with the green fluorescent protein (GFP) has enabled the direct visualization of real-time trafficking of GPCRs in living cells. Such analyses have provided crucial insight into the mechanisms involved in controlling GPCR function.
引用
收藏
页码:175 / 180
页数:6
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