DNA methylation controls histone H3 lysine 9 methylation and heterochromatin assembly in Arabidopsis

被引：372

作者：

Soppe, WJJ

Jasencakova, Z

Houben, A

Kakutani, T

Meister, A

Huang, MS

Jacobsen, SE

Schubert, I

Fransz, PF

机构：

[1] Inst Plant Genet & Crop Plant Res IPK, Dept Cytogenet, D-06466 Gatersleben, Germany

[2] Natl Inst Genet, Shizuoka 4118540, Japan

[3] Univ Calif Los Angeles, Dept MCD Biol, Los Angeles, CA 90095 USA

[4] Univ Calif Los Angeles, Inst Mol Biol, Los Angeles, CA 90095 USA

[5] Univ Amsterdam, Swammerdam Inst Life Sci, NL-1090 GB Amsterdam, Netherlands

来源：

EMBO JOURNAL | 2002年 / 21卷 / 23期

关键词：

Arabidopsis; DDM1; heterochromatin; histone methylation; MET;

D O I：

10.1093/emboj/cdf657

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We propose a model for heterochromatin assembly that links DNA methylation with histone methylation and DNA replication. The hypomethylated Arabidopsis mutants ddm1 and met1 were used to investigate the relationship between DNA methylation and chromatin organization. Both mutants show a reduction of heterochromatin due to dispersion of pericentromeric low-copy sequences away from heterochromatic chromocenters. DDM1 and MET1 control heterochromatin assembly at chromocenters by their influence on DNA maintenance (CpG) methylation and subsequent methylation of histone H3 lysine 9. In addition, DDM1 is required for deacetylation of histone H4 lysine 16. Analysis of F-1 hybrids between wild-type and hypomethylated mutants revealed that DNA methylation is epigenetically inherited and represents the genomic imprint that is required to maintain pericentromeric heterochromatin.

引用

页码：6549 / 6559

页数：11

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